Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

Figure 7

Antibodies anti-rNTHiENO recognize to native enolase of H. influenzae typeable and nontypeable. Immunodetection by western blot of the native enolase of H. influenzae using anti-rNTHiENO polyclonal antibodies. Lane M: prestained protein marker. Lanes 1-4: total protein extracts of HibBUAPNAN, HibATCC33, HiNTBUAP96, and HiNTBUAPPAU, respectively. Lanes 5-8: enriched membrane proteins, same order. Lane 9: rNTHiENO purified as positive control. The band corresponding to wild enolase (46.0 kDa) in H. influenzae strains is indicated with an arrowhead. The band corresponding to rNTHiENO (52 kDa) is indicated with an arrow.