Journal of Nucleic Acids / 2011 / Article / Fig 5

Review Article

Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA

Figure 5

Pseudouridine (Ψ-) specific reagents. (a) Pseudouridine and uridine reactivity with CMCT and similar water-soluble carbodiimides. Structural formula of CMCT is shown on the left. (b) Pseudouridine reactions with methylvinylsulfone and acrylonitrile. Pseudouridine residues are resistant to hydrazine, while unmodified uridines are specifically cleaved (see Figure 2(a)).

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