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Journal of Nanomaterials
Volume 2010, Article ID 196393, 6 pages
Research Article

Protein Viability on Au Nanoparticles during an Electrospray and Electrostatic-Force-Directed Assembly Process

1Department of Mechanical Engineering, University of Wisconsin-Milwaukee, 3200 North Cramer Street, Milwaukee, WI 53211, USA
2State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, The Institute for Advanced Materials and Nano Biomedicine, Tongji University, 1239 Siping Road, Shanghai 200092, China

Received 13 October 2009; Accepted 7 December 2009

Academic Editor: Chao Lin

Copyright © 2010 Shun Mao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We study the protein viability on Au nanoparticles during an electrospray and electrostatic-force-directed assembly process, through which Au nanoparticle-antibody conjugates are assembled onto the surface of carbon nanotubes (CNTs) to fabricate carbon nanotube field-effect transistor (CNTFET) biosensors. Enzyme-linked immunosorbent assay (ELISA) and field-effect transistor (FET) measurements have been used to investigate the antibody activity after the nanoparticle assembly. Upon the introduction of matching antigens, the colored reaction from the ELISA and the change in the electrical characteristic of the CNTFET device confirm that the antibody activity is preserved during the assembly process.