Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats
Figure 2
Emission spectra of 15 nM thiolated & nonthiolated oligo-TMR conjugates (copy number: 9.01 × 1012/mL) are sequentially shown under the following conditions: without any quencher (green & red curves); with 37.5 nM gold nanoparticles (2.25 × 1013 NPS/mL) (60% & 57% dynamically quenched: brown & blue curves) and with 37.5 nM gold nanoparticles plus 10 M MgCl2 (99% & 100% statically quenched: pink & green curves). Different emission peaks of gold-quenched thiolated & nonthiolated oligo-TMR conjugates are noted with brown & blue curves with emission peaks 579 nm and 585 nm, respectively.
