Figure 3: Activation of focal adhesion kinase (FAK) is observed on flat and ripples but not on wall structures. Confocal microscopy images show HMEC grown on the indicated substrate, fixed and immunostained with an antibody against the active form of FAK phosphorylated on Tyr397 (red). White square indicates the magnified part (lower panel, microscopic zoom in). Scale bars represent 10 μm. In Supplementary Figure 2 available online at http://dx.doi.org/10.1155/2013/251063, the images of Figure 3 are shown at enhanced brightness to visualize cell borders and the cell number.