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Journal of Nanomaterials
Volume 2013, Article ID 864374, 11 pages
Research Article

Fabrication of Novel Biodegradable α-Tricalcium Phosphate Cement Set by Chelating Capability of Inositol Phosphate and Its Biocompatibility

1Department of Applied Chemistry, School of Science and Technology, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki 214-8571, Japan
2Kanagawa Academy of Science and Technology (KAST), KSP East 404, 3-2-1 Sakado, Takatsu-ku, Kawasaki 213-0012, Japan
3Showa Ika Kohgyo Co., Ltd., 8-7 Hanei-nishimachi, Toyohashi 441-8026, Japan
4Department of Orthopaedic Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan

Received 8 April 2013; Accepted 8 May 2013

Academic Editor: Eng San Thian

Copyright © 2013 Toshiisa Konishi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Biodegradable α-tricalcium phosphate (α-TCP) cement based on the chelate-setting mechanism of inositol phosphate (IP6) was developed. This paper examined the effect of the milling time of α-TCP powder on the material properties of the cement. In addition, biocompatibility of the result cement in vitro using osteoblasts and in vivo using rabbit models will be studied as well. The α-TCP powders were ballmilled using ZrO2 beads in pure water for various durations up to 270 minutes, with a single-phase α-TCP obtained at ballmilling for 120 minutes. The resulting cement was mostly composed of α-TCP phase, and the compressive strength of the cement was  MPa, which suggested that the cements set with keeping the crystallite phase of starting cement powder. The cell-culture test indicated that the resulting cements were biocompatible materials. In vivo studies showed that the newly formed bones increased with milling time at a slight distance from the cement specimens and grew mature at 24 weeks, and the surface of the cement was resorbed by tartrate-resistant acid phosphatase-(TRAP-)positive osteoclast-like cells until 24 weeks of implantation. The present α-TCP cement is promising for application as a novel paste-like artificial bone with biodegradability and osteoconductivity.