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Journal of Nanomaterials
Volume 2015, Article ID 391850, 6 pages
Research Article

Preparation of Homogeneous Nanostructures in 5 Minutes for Cancer Cells Capture

1Department of Oncology, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu 210003, China
2The Fourth Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu 210009, China
3Department of Radiation Oncology, The Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, China

Received 30 December 2014; Revised 28 March 2015; Accepted 31 March 2015

Academic Editor: Yaling Liu

Copyright © 2015 Lixue Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Grafting aptamers on nanostructured substrates has shown ultrasensitivity in isolation of circulating tumor cells (CTCs). Here, we report that over 80 cm2 of homogenous nanostructured surface on glass substrates can be prepared in 5 min after one-step dry etching. The surface area was doubled; the average diameter of nanostructures is approximately 374 nm, which is more close to the nanostructures of natural extracellular matrix. Antiepithelial cell adhesion molecule aptamers grafted nanostructured glass substrates captured over 76% of PC3 cells compared to 30% of planar substrates. Bispecific aptamers cofunctionalized nanostructured substrates, however, fail to capture cancer cells probably due to the formation of heterodimers. This limitation reveals that multispecific aptamers, when applied to cell isolation, must be analyzed to exclude any potential formation of heterodimers due to complementary sequence matching.