COX-2 Regulates the Proliferation and Apoptosis of Activated Hepatic Stellate Cells through CDC27

Figure 5

Effects of shRNA-mediated COX-2 knockdown on HSCs related with the changed expression of CDC27. (a) Western blotting for CDC27 protein expression in all groups. (b) Cell proliferation was detected in cotransfection of COX-2 and CDC27 shRNA cells, COX-2-shRNA-1 cells, and sh-NC cells. (c) HSC-T6 cells were transfected with COX-2-shRNA-1, or sh-NC, or cotransfected with COX-2-shRNA-1 and sh-CDC27. Cell cycle analysis was examined by flow cytometry. (d) Apoptosis of HSC-T6 cells transfected with COX-2-shRNA and CDC27-shRNA, COX-2 nanomedicine-shRNA-1, and sh-NC was examined by flow cytometry. and , compared with the sh-NC group and COX-2-shRNA-1 group.

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