Hypercholesterolemia can be a consequence of excessive dietary saturated fatty acid (SFA), while almond-supplemented diets can improve lipid profiles. However, the differential and independent impacts of dietary SFA and almondsupplemented diets on plasma total cholesterol (pTC) and low-density lipoprotein (pLDL-c) concentrations have not been directly compared and are not well described. We reviewed the available data to construct multiple regression analyses to theoretically assess the impact of relative almond intake (RAI) and dietary SFA on reducing pTC and pLDL-c concentrations. Strong, negative correlations between RAI and percent change in mean pTC and RAI and percent change in mean pLDL-c were detected. The relationships between percent change in mean dietary SFA, and percent change in mean pTC and mean pLDL-c were weaker and only significant for pLDL-c. The multiple regression analyses demonstrated modest improvements in the strength of the correlations for both pTC and pLDL-c . The models suggest that the increase in RAI contributes to the reduction in pTC and pLDL-c to a greater extent than a reduction in dietary SFA, but a simultaneous decrease in dietary SFA should further improve lipid profiles.

1. Introduction

Chronic hypercholesterolemia is a critical risk factor in the development of cardiovascular disease (CVD), which is the most common cause of death worldwide [14]. Furthermore, low-density lipoprotein (LDL) is the major atherogenic lipoprotein and the primary target of cholesterol-lowering therapy because numerous clinical trials have demonstrated the efficacy of LDL-lowering therapy for reducing the risk of CVD [3, 4]. A recognized consequence of increased dietary saturated fatty acid (SFA) is hypercholesterolemia [511]. Conversely, diets supplemented with almonds or almond products (i.e., oil and butter) have been shown to produce a moderate, yet significant decrease in plasma total cholesterol (pTC) (3–11%) and plasma LDL cholesterol (pLDL-c) (3–18%) [1223], which demonstrates a potential benefit from consuming almonds on improving cardiovascular health. For these reasons, studies of natural foods that have the potential to significantly improve circulating lipid profiles, especially reducing pLDL-c, are of particular importance. The nutriceutical benefits of nuts provide promise for taking a dietary approach to addressing the increasing prevalence of CVD globally. The mechanisms by which nuts and nut-supplemented diets contribute to reduced pTC and pLDL-c have not been revealed, and, given the nature of these types of studies, elucidation of these mechanisms in humans is not likely. Thus, the intriguing question of how nuts induce a cholesterol-lowering benefit remains. Is the effect simply and strictly displacement or do nuts reduce de novo cholesterol synthesis? In the interim, theoretical studies that provide a better understanding of the effects of almond-supplemented diets on plasma cholesterol will serve a meaningful purpose to this end and provide further insight on the impacts of dietary interactions among different foods.

A modeling approach to better understand the impacts of dietary fats and nut consumption on plasma cholesterol has been realized [24]. This highly innovative approach and significant contribution to the area of nut consumption and circulating lipids examined the effects of substituting saturated fat intake with monounsaturated and polyunsaturated fatty acids by varying the consumption of various nuts [24]. This study acknowledged that the levels of dietary SFA may be manipulated by the consumption of nuts, which is an effective strategy for reducing pLDL-c concentrations, and for preventing a reduction in HDL-cholesterol and an increase in plasma triglyceride induced by low fat, high carbohydrate diets [24]. However, this comprehensive and elegant meta-analysis examined all nuts and did not specifically focus on assessing covariate effects of dietary SFA and almond supplementation on changes in plasma cholesterol. Furthermore, we took an alternative approach to assessing almond consumption by examining consumption as a function of body mass. Most studies report nut consumption as a fixed variable without consideration for a potential effect of changes in body mass, which is a tenet of pharmacological studies. That is, we wanted to evaluate if a dose-dependent effect of almond consumption on plasma cholesterol (TC and LDL-c) existed, which has not been presented previously. Thus, the current study was conceived in a manner to complement the significant contributions of those previously described [24]. Therefore, we modeled the more recent data on the effects of almond-supplemented diets on plasma cholesterol to address the hypothesis that relative almond intake has a greater impact on reducing plasma cholesterol than dietary SFA.

2. Methods

A PubMed search for peer-reviewed publications on the effects of almonds and almond-supplemented diets on plasma cholesterol was conducted. Twenty-one studies were found that reported on the effects of diet and almond supplementation on pTC, and pLDL-c levels. While each study implemented different diets, the present analyses were based on reported mean values for the amount of almonds consumed, body mass (BM), dietary SFA, pTC and pLDL-c. Thus, these inclusion criteria had to be reported in a manner that percent changes in mean dietary SFA, pTC, pLDL-c, and relative almond consumption (RAI; almond intake as a function of body mass) between initial and final measurement periods could be calculated [12, 1822] with one exception [15].

2.1. Relative Almond Intake

Because the amount of almond supplementation within a particular study is fixed, despite differences in BM of participants, almond consumption across studies was normalized to account for these differences in mean BM. Thus, RAI was calculated and presented as g/kg BM. In theory, this approach should help alleviate the impact of the differences in mean BM among the study subjects and also help normalize for differences in almond doses used among the different studies. Because Hyson et al. [15] reported BMI and not BM, BM was derived from mean BMI assuming an average height for their study population of 1.7526 m (69 in) given that more women () than men () comprised their study population. Furthermore, we calculated RAI using a range of assumed heights ( in) and the, at most, 8% difference in RAI (0.84 g/kg BM) did not significantly alter the regression values.

2.2. Change in Dietary Saturated Fatty Acids

The change in mean dietary SFA was calculated as percent change from baseline to account for the differences in how values were reported (i.e., percentage of energy or g/d).

2.3. Changes in Plasma Total Cholesterol and Low-Density Lipoprotein

Similarly, because pTC and pLDL-c were reported in both standard (mg/dL) and metric (mM) units among the different studies, this difference was accounted for by calculating percent change in pTC and pLDL-c between initial and final measurement periods in each study.

2.4. Statistical Analyses

Relative almond consumption was identified as a contributing factor to a reduction in pTC and pLDL-c. The effect of dietary SFA intake on pLDL-c was moderately strong , but significant () and the effect on pTC was similarly as strong , but only borderline nonsignificant (). Thus, each factor alone was not able to sufficiently account for the changes in plasma TC or LDL-c. Therefore, a multivariate regression model was used where denoted relative almond intake, denoted the percent change in dietary SFA, and denoted the percent change in plasma TC or LDL-c. The multivariate, linear regression model used to fit the data was defined by The coefficients , , and were computed using ordinary least squares applied to the data collected. The coefficient of determination or value was computed to assess the validity of this multivariate, linear regression model. Scatter plots of the data provided a method to qualitatively compare the model to the data. Moreover, 2 data points (control group from Spiller et al. [22] and raw-almond group from Tovar et al. [9]) were identified as statistical outliers and not considered in the regression models here. The slopes of the regression analyses were compared by analysis of covariance (ANCOVA). Regressions and slopes were considered significant at . Models were constructed and means compared using STAT software (version 3.0; Richmond, VT).

3. Results

3.1. Relative Almond Intake and Plasma Total Cholesterol and Low-Density Lipoprotein

The compiled analyses demonstrated a strong , negative, and significant () relationship between percent change in mean RAI and percent change in mean pTC (Figure 1(a)). The relationship between percent change in mean RAI and percent change in mean pLDL-c was slightly stronger , but more significant () than that for percent change in mean pTC (Figure 1(b)). The slopes were not different () between the two relationships.

3.2. Dietary SFA Intake and Plasma Cholesterol and Low-Density Lipoprotein

While a moderately strong, positive relationship between percent change in dietary SFA intake and percent change in mean pTC was detected, this relationship was not statistically significant (). As with the relationship between RAI and pLDL-c, the relationship between percent change in dietary SFA intake and percent change in mean pLDL-c was stronger than that for pTC and was significant (). The slopes were not different () between the two relationships.

3.3. Multiple Regression Analyses

To more thoroughly evaluate the effects of multiple factors known to impact pTC and pLDL-c, multiple regression analysis was performed. These analyses demonstrated strong and significant relationships between the independent variables percent change in mean RAI and percent change in mean dietary SFA intake) and percent change in mean pTC (Figure 2) or percent change in mean pLDL-c (Figure 3), with the effects greater on pLDL-c than on pTC.

4. Discussion

Hypercholesterolemia continues to serve as the most predictive risk factor for the development of cardiovascular disease (CVD) [14, 25], and because LDL-c constitutes a majority of total circulating cholesterol, pLDL-c is the primary target for cholesterol-lowering therapies [3, 4, 25]. In addition to genetic factors that contribute to hypercholesterolemia [26], excessive consumption of dietary SFA may also contribute to increased plasma cholesterol (total and LDL) levels [11]. A variety of nuts (almonds, walnuts, pistachios, peanuts, and macadamia nuts) have been reported to possess cholesterol-lowering benefits [5, 6, 810, 1324, 2734] suggesting that dietary modifications have the potential to improve lipid profiles and ultimately abate the prevalence of CVD. Therefore, a more thorough analysis of the theoretical relationships among dietary SFA, almond consumption, and plasma cholesterol could prove worthwhile in assessing the potential benefits of dietary modifications.

The most significant finding of the present analyses suggests that increased almond consumption has theoretically a greater potential to reduce both plasma total and LDL cholesterol than reduced dietary saturated fatty acids alone. This is corroborated by the fact that strong and highly significant relationships between RAI and pTC and pLDL-c were detected. Conversely, the relationships between percent change in mean dietary SFA and pTC and pLDL-c were only moderately strong, and only significant for pLDL-c. Along these lines, the impact of increased RAI was greater on reducing percent change in mean pLDL-c than on mean pTC suggesting that the benefits of increased almond consumption on lowering cholesterol could be therapeutic because of their effectiveness on the primary target (LDL-c) of pharmacological interventions. While it is likely that an increase in sample size will lead to a significant relationship between percent change in dietary SFA and pTC, the strength of the relationships between RAI and both pTC and pLDL-c were consistently stronger than those with dietary SFA suggesting that RAI has a greater effect on pTC and pLDL-c than reduced dietary SFA intake. The multiple regression analyses demonstrate that the inclusion of the dietary SFA data in the analyses only modestly improves the strength of the regressions for pTC and pLDL-c further suggesting that increased RAI is the primary contributor to the reductions in mean pTC and pLDL-c. Nonetheless, reducing dietary SFA in addition to increasing almond consumption has a greater potential for reducing plasma cholesterol (total and LDL) than either has alone. Alternatively, the results also suggest that increased or the lack of a reduction in dietary SFA intake may impair the ability of almonds to reduce plasma cholesterol levels. Thus, to realize the greatest benefit of the consumption of almonds, and possibly other nuts, on plasma total and LDL cholesterol, a simultaneous reduction in dietary SFA intake would be recommended.

As is the case with most theoretical studies, we are cognizant of limitations in the approach and interpretations. Because most of the studies we reviewed targeted reducing pTC and pLDL-c as principal outcomes, it should not be completely unexpected that the impact of increased RAI was greater than reduced dietary SFA on pTC and pLDL-c. Also, comparing the effects of both independent variables is complicated because studies reviewed here could not completely control for changes in dietary SFA as some studies tried to displace dietary SFA with almond fats. Future studies where dietary SFA and almond fats can be better controlled will help alleviate this limitation. But this limitation is more likely a factor of study participant compliance than actual study design, so this issue may linger in future studies. The inclusion of only 7 out of 21 papers identified also limits the impact of these findings. Additionally, because so few studies exist on the effects of other nuts on lipid profiles such theoretical analyses are limited to almonds. However, comparisons among different nuts would be interesting to determine if nuts as a group are equivalent in terms of their effects on blood lipid profiles. Nonetheless, given these limitations, the fact that significant relationships could be detected suggests that almonds, and possibly other nuts, have a practical effect on improving plasma cholesterol, largely independent of reductions in dietary SFA.

5. Conclusions

The ramification of the calculated relationships is that the benefits of almond consumption on reducing plasma total and LDL cholesterol are greater than reduced dietary SFA intake alone. While we recognize and demonstrate the importance reduced dietary SFA has on reducing plasma total and LDL cholesterol, the present estimations suggest that increased almond intake is more beneficial. Furthermore, the nature of the relationships would suggest that the cholesterol-lowering effects of almonds are, at least in part, a function of displacement. Future animal studies designed to specifically address the effects of almonds on displacement versus de novo synthesis would be impactful and highly informative. Nonetheless, if the relationships are truly linear, then the greater the reduction in dietary SFA intake, the more impactful the cholesterol-lowering effects of almonds should be. How therapeutic these benefits are at ameliorating CVD requires further investigation, but these analyses provide legitimacy for the pursuit of such studies. Moreover, it would be interesting to learn if the benefits of RAI on pTC and pLDL-c are saturable so that an upper-limit of almond intake can be identified with respect to optimizing the benefit on reducing plasma cholesterol. Furthermore, a study that simultaneously controls for both relative almond consumption and dietary SFA intake would provide further insight on the contribution of each variable to reducing plasma cholesterol. Whether or not a similar relationship can be established for other nuts or foods would be interesting and could be beneficial when producing dietary recommendations for those particular foods, especially when considering dietary SFA.

Conflict of Interests

None of the authors have any conflict of interests to disclose.


The authors would like to thank Dr. S. A. Adams (USDA, WHNRC) for insightful comments and discussion of this topic during its inception. The authors, acknowledge and appreciate the feedback on an earlier draft of this paper by Drs. M. Dreher and K. Lapsley. The project idea was developed while R. M. Ortiz was an E. (Kika) de la Garza Fellow (USDA). R. M. Ortiz was partially supported by NIH NHLBI K02HL103787 during the writing of this paper.