Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase

Figure 1

Peroxidation of pyrogallol catalyzed by free CPO (open squares; 0.575 μg CPO in 3 mL total assay volume) and sol-gel entrapped CPO (grey circles; 0.575 μg CPO entrapped in three sol-gel beads placed in 3 mL total assay volume). The CPO sol-gel beads were prepared from TMOS with a pH 6.0 casting buffer and matured for one week with a buffer exchange on every other day. The activity assays were performed in 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2 with constant concentrations of either 2.7 mM H₂O₂ (a) or 35 mM pyrogallol (b). All data were measured in triplicate and are displayed as mean values ± one standard deviation. The parameters of the curve fits are summarized in Table 1.

(a)

(b)