Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment
Table 1
Thermal profile for amplification of the used bacteria.
Name of the bacteria
Target gene
Thermal profile
Amplicon size (bp)
Reference
Bacillus spp.
16s rRNA gene
Initial denaturation at 94°C for 5 minutes, followed by 30 cycles of denaturation at 95°C for 30 seconds, annealing at 55°C for 20 seconds, and extension at 72°C for 30 seconds. The final extension was conducted at 72°C for 7 minutes
Initial denaturation at 95°C for 2 minutes, followed by 25 cycles of denaturation at 94°C for 20 seconds, annealing at 54°C for 20 seconds, and extension at 72°C for 40 seconds. The final extension was conducted at 72°C for 1 minute