Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

Table 1

Thermal profile for amplification of the used bacteria.

Name of the bacteria	Target gene	Thermal profile	Amplicon size (bp)	Reference
Bacillus spp.	16s rRNA gene	Initial denaturation at 94°C for 5 minutes, followed by 30 cycles of denaturation at 95°C for 30 seconds, annealing at 55°C for 20 seconds, and extension at 72°C for 30 seconds. The final extension was conducted at 72°C for 7 minutes	463	[80]
Pseudomonas spp.	16s rDNA gene	Initial denaturation at 95°C for 2 minutes, followed by 25 cycles of denaturation at 94°C for 20 seconds, annealing at 54°C for 20 seconds, and extension at 72°C for 40 seconds. The final extension was conducted at 72°C for 1 minute	618	[81]