α2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5
Figure 7
The α2 ectodomain regulates expression of KLK-5, 6, and 7, the combination of which mediates an anti-invasion phenotype in vitro. (a) (i) CollagenI adhesion of MP2-mock and MP2-α2/TC cells compared with MP2-α2Δ cells. (ii) Invasion analysis of MP2-mock versus MP2-α2Δ cells (left panel), or MP2-mock versus MP2-α9α2 and MP2-α9DM1 cells (right panel). (b) (i) Heatmap presentation of the probeset shown in Figure 4(a)(i) in MP2-α2Δ and MP2-α9α2 cells. (ii) RT-PCR verification of KLK-5, 6 and 7 expression in MP2-α2Δ and MP2-α9α2 cells. (c) (i) Invasion of the indicated cells in the present or absence of function-blocking anti-α2 antibody. (ii) Model of PDAC invasion status based on α2-dependence and KLK5 expression status.
