Journal of Oncology / 2012 / Article / Fig 2

Research Article

Chronic Myelogenous Leukemia Cells Contribute to the Stromal Myofibroblasts in Leukemic NOD/SCID Mouse In Vivo

Figure 2

Analysis of the engrafted CML cells in the transplanted NOD/SCID murine bone marrow. (a) RT-PCR analyses of the indicated cells. Bcr-Abl was analyzed for the detection of fusion molecule; CD13 and CD33, human myeloid markers that are also expressed in the injected CML cells; CD34 and CD133, human stem cell markers that are expressed in the injected CML cells; CD106 and FSP1, human stromal markers that are expressed in human bone marrow adherent cells; GAPDH, RT-PCR control. MW indicates molecular weight marker; BM, bone marrow; CML whole, bone marrow mononuclear cells from CML patient with no processing; Fib, myofibroblasts. (b) Appearance of the spleen from the control NOD/SCID mouse and from human CML cell-engrafted NOD/SCID mouse. (c) CD34 and CD133 expressions of the CML cell-engrafted NOD/SCID murine bone marrow. (d) Expression of CD13 and CD33 after the engrafted NOD/SCID murine bone marrow cells were separated with anti-human CD34 Ab-coated magnetic beads. (e) Morphology of NOD/SCID murine bone marrow cells after engraftment of human CML cells. Cells were stained with May-Giemsa solution (×400, ×1000).
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