Chronic Myelogenous Leukemia Cells Contribute to the Stromal Myofibroblasts in Leukemic NOD/SCID Mouse In Vivo
Figure 3
Characteristics of myofibroblasts from CML cell-engrafted NOD/SCID murine bone marrow cells. (a) Expression of CD106 and CD133. After selected with anti-human CD34 Ab-coated magnetic beads cells were further separated with anti-human D7-FIB Ab-coated magnetic beads, and analyzed. (b) Microscopical appearance of the separated myofibroblasts from CML cell-engrafted NOD/SCID murine bone marrow cells (×400). May-Giemsa, and immunocytochemical staining of S100, human fibronectin, and human SMA. (c) FISH analysis of the separated myofibroblasts from CML cell-engrafted NOD/SCID murine bone marrow. BCR-ABL fusion signal is indicated with an arrow. 83 cells are positive in total 100 cells analyzed.
