Survivin Regulates Bad Gene Expression by Binding to Its Promoter and Modulates Cell Cycle and Apoptosis in Esophageal Carcinoma Cell

Figure 1

The effects of survivin overexpression and knockdown on the expression of Bad, caspase-3, and caspase-7 gene. The expressions of survivin, Bad, caspase-3, and 7 were determined by qRT-PCR and confirmed by Western blot. GADPH served as an internal and loading control. (a) When survivin was overexpressed in KYSE450 cells, Bad ( < 0.05) and caspase-3 ( < 0.05) were downregulated. (b) When survivin was overexpressed in ECA109 cells, Bad ( < 0.01) was downregulated. (c) When survivin was downregulated in KYSE450 cells, Bad ( < 0.01) was upregulated, but there were no significant differences in caspase-3 ( > 0.05) and caspase-7 ( > 0.05). (d) When survivin was downregulated in ECA109 cells, Bad ( < 0.01) was upregulated, but there were no significant differences in caspase-3 ( > 0.05) and caspase-7 ( > 0.05) (Fig 1), compared to BC and NC groups. Data are presented as the means ± standard deviation from triplicate experiments. BC: blank control; NC, UP, and KD: cells in these groups were transfected with negative control, overexpression, and shRNA plasmid, respectively. Statistically significant differences compared to BC and NC groups are indicated ( < 0.05,  < 0.01).

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