Chidamide and Radiotherapy Synergistically Induce Cell Apoptosis and Suppress Tumor Growth and Cancer Stemness by Regulating the MiR-375-EIF4G3 Axis in Lung Squamous Cell Carcinomas

Figure 2

Chidamide or chidamide combined with radiation treatment suppressed cancer stemness in NCI-H2170 and NCI-H226 cells. (a–j) Sphere formation assay to determine the self-renewal capacities of NCI-H2170 and NCI-H226 cells treated with chidamide alone or a combination of chidamide and radiation. The sphere sizes and number were calculated in Figures 2(c)–2(j). (k–m) Treated cells with chidamide alone (300 nM) or combined with radiation treatment were subjected to ALDEFLUOR assay and the population of ALDH⁺ cells were counted by flow cytometry. Statistical significance was determined by Student’s t-test and indicated by ; ; and (vs. control); ; (vs. Gy).

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