Chidamide and Radiotherapy Synergistically Induce Cell Apoptosis and Suppress Tumor Growth and Cancer Stemness by Regulating the MiR-375-EIF4G3 Axis in Lung Squamous Cell Carcinomas

Figure 7

EIF4G3 is a direct target of miR-375. (a) Schematic view to present putative miR-375 binding sites in the 3′UTR region of EIF4G3. The mutant sequences are shown below. (b) Luciferase reporter assay to assess the effect of miR-375 on the transcription of Renilla luciferase with 3´UTR of EIF4G3 expression. Data were shown as means ± SEM. Each group was performed in six biological replicates. ; . (c) Western blot analysis to determine the protein expression of EIF4G3 in NCI-H2170 and NCI-H226 cells transfected with miR-375 mimic at the concentration of 0 nM, 50 nM, and 100 nM, respectively. (d)-(e) Detection of the mRNA expression of EIF4G3 in NCI-H2170 and NCI-H226 cells with indicated treatment. (f) Western blot analysis to detect the expression of EIF4G3 in the indicated groups. (g–i) The apoptosis rate of NCI-H2170 and NCI-H226 cells transfected with 100 nM of EIF4G3 siRNA, compared with control. (j–l) EIF4G3 inhibition significantly suppresses tumor growth in vivo in NCI-H2170 cells. Tumor growth curves and tumor weight analysis are shown in Figures 5(h) and 5(i), respectively. All the data were statistically analyzed by Student’s t-test. ; ; and .

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