PLCD1-Induced DNA Damage Inhibits the Tumor Growth via Downregulating CDKs in Chondrosarcoma

Figure 5

PLCD1-induced DNA damage caused cell apoptosis in vivo and in vitro. (a) Western blotting revealed DNA damage response protein upregulated by PLCD1 overexpression. (b) Flow cytometry analysis revealed cell apoptosis in OE-PLCD1 cell lines in vitro. (c) Significant increase of cells in the sub-G1 phase suggests a cell death population according to Figure 3(f). (d) Western blotting revealed cell apoptosis protein upregulated by PLCD1 overexpression. (e) TUNEL assay kit of OE-PLCD1 cell line in vivo. (f) Rescue experiment of cell apoptosis induced by PLCD1 overexpression.

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