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Journal of Ophthalmology
Volume 2015, Article ID 152854, 7 pages
http://dx.doi.org/10.1155/2015/152854
Research Article

Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways

1Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan
2Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan
3Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan
4Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA
5Department of Optometry, Yuan Pei University, Hsinchu 30015, Taiwan

Received 9 September 2015; Accepted 15 October 2015

Academic Editor: Qing-huai Liu

Copyright © 2015 Shih-Chun Chao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01–1 μg/mL) and lutein and zeaxanthin (1–10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.