Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea

Figure 2

Neovascularization in corneal stroma as observed in whole-mounted specimens. We first observed the morphology of neovascularization in whole-mounted specimens by employing CD34 immunostaining. WT ((a), (b)) and KO (c) corneas at day 3 show loop-like distribution of blood limbal vasculature (arrows in (a), (b), and (c)). In WT corneas neovascularization was observed in the stroma apart from the limbus (arrowheads in (a) and (b)), although the staining procedure did not figure the continuous elongation of the vessels. Dotted lines, limbal corneoscleral border; bar, 100 μm.

(a)

(b)

(c)