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Ferdinando Bottoni, "Fundus Autofluorescence in Lamellar Macular Holes and Pseudoholes: A Review", Journal of Ophthalmology, vol. 2019, Article ID 4948212, 7 pages, 2019. https://doi.org/10.1155/2019/4948212
Fundus Autofluorescence in Lamellar Macular Holes and Pseudoholes: A Review
Macular pseudoholes (MPHs) and lamellar macular holes (LMHs) have been recently defined according to spectral domain optical coherence tomography (SD-OCT) criteria. A major feature for differentiating an MPH from an LMH remains the loss of foveal tissue. The anatomy of the foveola is peculiar with the macular pigment (MP) embedded in a very thin layer of tissue underlying the internal limiting membrane and mainly constituted of a specialized group of Müller cells and Henle’s fibers. Despite the near microscopic resolution (≈5–7 μm) and the capability to visualize the outer retina in detail, SD-OCT may fail to ascertain whether a very small loss of this foveolar tissue has occurred. Blue-fundus autofluorescence (B-FAF) imaging is useful in this respect because even very small loss of MP can be identified, suggesting a corresponding localized loss of the innermost layers of the foveola. A definition of MP loss would help differentiating an LMH from an MPH where B-FAF imaging will be negative.
Macular pseudoholes (MPHs) and lamellar macular holes (LMHs) have been defined in many different ways [1, 2], but the common denominator has always been whether or not a loss of foveal tissue is present. Even in the last classification by the International Vitreomacular Traction Study Group , MPHs are characterized by the absence of foveal tissue loss, whereas in LMHs, a partial defect of the inner fovea should be present.
Clinically, by fundus examination, both MPHs and LMHs have a similar appearance with a round and well-circumscribed reddish lesion at the center of the fovea [1, 4–11]. Functional tests, such as the Watzke-Allen test  and microperimetry , in which no scotoma is detected in either MPH and LMH, are not useful for differentiating between these conditions, and both clinical entities can lead to similarly impaired vision (median 20/40) [1–4].
Time domain (TD) [1, 7–11, 14] and spectral domain (SD) [2, 3, 15, 16] optical coherence tomography (OCT) have expanded our knowledge about pathogenesis, morphology, and progression of these clinical entities. However, despite their near-microscopic resolution (≈5–7 μm) and the capability to visualize the outer retina in detail, SD-OCT may fail to ascertain whether a very small loss of foveal tissue has occurred. Blue-fundus autofluorescence (B-FAF) imaging is useful in this respect, especially in early LMHs when there is a lack of evident break in the inner fovea and dehiscence of the retina [2, 14], and the diagnosis of MPHs or LMHs remains a matter of speculation.
The pathogenesis of MPHs and LMHs is not fully understood. It has been hypothesized that an MPH is attributable to the centripetal contraction of an epiretinal membrane . In contrast, an LMHs is thought to be the result of an abortive process in the formation of an FTMH. Posterior vitreous detachment is the main initiating process of the latter, but epiretinal membrane contraction has been suggested as a likely secondary factor [2, 16, 17]. This mechanism is also supported by two findings: (i) 62% to 89% of LMHs may present with an epiretinal membrane [1, 2]; and (ii) pseudo-opercula, suggestive of an aborted macular hole, have been reported in only 24% of patients with LMH . Therefore, it seems that the pathogenesis of LMH cannot simply be attributed to an abortive anteroposterior traction, the classic process in FTMH formation.
In a normal fundus, the distribution of fundus autofluorescence (AF) is diffuse, with decreased intensity at the optic nerve head, the retinal blood vessels, and the macula [18, 19]. Macular AF is attenuated by the presence of luteal pigment, which has a very high density in the fovea (Figure 1(a)). Originally, the foveolar macular pigment was thought to be located in the Henle fiber layer (Figure 1(b)) . It has been recently demonstrated that (in addition to the typical “z-shape” Müller cells of the fovea) there are 25–35 unique Müller cells in the foveola . These Müller cells are suggested to form the “Müller cell cone,” i.e., the inverted cone-like structure that overlies the area of high photoreceptor density in the primate foveola . Inner processes and nuclei of these specialized Müller cells together with the axons of the highly packed foveal cones constitute the very thin layer of tissue immediately below the foveolar internal limiting membrane (ILM) (Figure 1(c)); that is, the place where the foveolar macular pigment is located at high density . The density of the macular pigment decreases continuously from the center to the periphery of the foveola. The stalk of the “Müller cell cone” as well as Müller cell processes in the nerve fiber layer, inner plexiform layer (IPL), outer plexiform layer (OPL), and Henle fiber layer (HFL) in the central fovea walls also contain the macular pigment, albeit at lower density [20, 21]. Any foveal defect, including an LMH that spares the photoreceptors , may alter the degree of foveal AF by decreasing the amount of masking luteal pigment and thus increasing the foveal AF.
4. Imaging Techniques
4.1. Slit-Lamp Biomicroscopy
Slit-lamp biomicroscopy in patients with MPHs and LMHs may simply show the common feature of a round, reddish lesion at the center of the macula, but it is not sensitive enough to detect a small loss of foveal tissue, which is present in LMHs with preserved visual acuity. Additionally, the presence of an epiretinal membrane is not definitive in the differential diagnosis of LMH and MPH; as mentioned above, 62% to 89% of patients with LMH may have an associated epiretinal membrane [1, 2], which will always be present in MPH .
Haouchine et al.  showed that only 28% of LMH cases diagnosed with OCT were diagnosed as LMH on fundus examination. Likewise, Witkin et al.  reported that only 37% of LMH cases diagnosed using ultrahigh-resolution OCT were detected clinically on fundus examination. These data show the limitation of slit-lamp biomicroscopy in the diagnosis of LMH.
4.2. Optical Coherence Tomography
The criteria for the OCT diagnosis of MPH and LMH were originally defined by Haouchine et al.  and Witkin et al.  and subsequently confirmed by the International Vitreomacular Traction Study Group .
The four basic criteria for the OCT diagnosis of LMH are an irregular foveal contour, a break in the inner fovea, a dehiscence of the inner foveal retina from the outer retina, and an absence of a full-thickness foveal defect with intact foveal photoreceptors. Further confusion was added to the debate by a later assertion that LMH with lamellar cleavage of their edge remain pseudoholes because there is no loss of foveal tissue as shown by SD-OCT . It is clear that one of the major problems encountered in an OCT diagnosis of a foveal defect is the difficulty of determining with certainty whether there is loss of retinal tissue. Furthermore, if there is loss of foveal tissue, it is difficult to determine its anatomic location. The reason for that became apparent looking at the last SD-OCT classification of posterior ocular layers by the International Nomenclature Panel  who took into consideration the findings on Henle’s fibers imaging [24, 25]. As already mentioned, the foveal center is made of a thick photoreceptor layer and a specialized group of Müller cells ; Henle’s fibers are reduced to an extremely thin layer (Figure 2(a)). The entry position of the SD-OCT beam through the pupil must vary to reliably identify Henle’s fibers temporal and nasal to the fovea [24, 25] (Figures 2(b) and 2(c)). The acquired images correlate beautifully with histologic specimens (Figures 2(a)–2(d)), but the inner foveolar layers remain difficult to visualize in normal eyes (Figures 2(b)–2(d)). If a LMH is suspected (i.e., loss of foveal tissue), it is even more difficult to determine whether or not this thin layer of tissue under the foveolar ILM containing the macular pigment is missing (Figures 3(a) and 3(c)).
Therefore, because of the peculiar reflectivity of the innermost retinal layers in the foveola, OCT imaging might not be sensitive enough to detect early loss of foveal tissue, that is, an initial LMH.
4.3. Fundus Autofluorescence
Fundus autofluorescence (AF) has challenged the current OCT concepts regarding the differentiation between MPH and LMH [1–3, 5, 14, 16]. Thus, foveal lesions with SD-OCT features of MPH (i.e., irregular foveal contour with steep edges and near-normal central foveal thickness with apparent no loss of tissue) often demonstrate increased foveal AF (Figures 4(a) and 4(b)). It was already reported in a study of patients with residual retinal tissue at the bottom of the fovea classified as MPH and LMH according to the OCT profiles established by Haouchine et al.  that the foveal AF intensity did not differ between these two conditions , where increased foveal AF was found. It could be argued that increased foveal AF could be the result of dislocated macular pigment due to the tangential traction in the fovea exerted by an epiretinal membrane. There are two reasons that seem to oppose this hypothesis: first, the lack of increased AF signal in patients with idiopathic epiretinal membrane and macular pucker (Figures 5(a) and 5(b)). Second, the presence of increased foveal AF signal in eyes with an abnormal foveal contour on OCT and no signs of tangential traction in the macula (Figures 6(a) and 6(b)). Lipofuscin-laden RPE generates fundus AF , which in the macula is attenuated by the luteal pigment. Most of this pigment in the foveola resides below the ILM, among inner processes/nuclei of specialized Müller cells and axons originating from the foveolar cones . It has been recently demonstrated that in eyes with LMHs, a strong correlation exists between the diameters of the holes measured with B-FAF and those measured at the OPL level with OCT . This may indicate that indeed a loss of foveolar tissue containing the macular pigment at the OPL level in the Müller cell inverted cone is likely.
Thus, even very thin foveal defects, such as those affecting only the innermost part of the foveola sparing the photoreceptors, as in initial LMH, may increase the foveal AF. Therefore, AF findings (i.e., increased AF at the fovea) in patients with MPH and LMH as defined by SD-OCT suggest that in both conditions there could be a loss of foveal tissue.
5. Clinical Implications of AF Findings
The lack of a significant difference in foveal AF between LMH and MPH, as diagnosed by means of OCT imaging, raises questions concerning the validity of this OCT differentiation. If the loss of foveal tissue is considered for the diagnosis of LMH, it must be acknowledge that determining a very thin loss of tissue in the foveola may be difficult using a SD-OCT. There are two options for ascertaining such a type of loss: A direct way would imply a direct visualization of the tissue loss by OCT, with the previous limitations underlined above. An indirect way would be to determine the loss of macular pigment, which is located in the “Müller cell inverted cone” in the foveola, by means of fundus AF. An absence or decrease of the macular pigment would increase foveal AF. The anatomical localization of the macular carotenoid pigments is a complex and controversial subject with evidence suggesting that there is a pigment in both the Müller cells and the cone axons of the fovea. As Snodderly beautifully showed (Figure 1(b)) , the macular pigment is present not only under the foveal ILM but also deeper toward the foveal cone fibers and in the outer and inner plexiform layers. Carotenoid pigments have been demonstrated in surgically removed lamellar hole-associated epiretinal proliferation (LHEP) which is supposed to be constituted, at least in part, by Müller cells . Syrbe et al. , more recently, could not ascertain with certainty the exact location of the macular pigment if not confirming that it is inside the Müller cell inverted cone. That is an important clue for diagnosing tissue loss in the fovea because an increased B-FAF in the fovea would imply the absence of a masking element that should be normally there.
Whether or not this can be used for establishing a differential diagnosis between LMH and MPH remains to be further investigated. However, given the uncertainty encountered in the direct diagnosis of foveal tissue loss with OCT, B-FAF imaging may be of help as to this purpose.
An accurate diagnosis of FTMH, LMH, and MPH is important to determine the proper surgical treatment of these lesions. Different options may be selected according to the OCT and fundus AF imaging findings. For instance, in the absence of foveal AF, the integrity of the foveal tissue is almost certainly confirmed. Therefore, it is likely that removal of the epiretinal membrane alone is all that is needed in such cases. However, if foveal AF is present, a loss of foveal tissue has very likely occurred, and the decision to operate will depend on many factors, such as the residual visual acuity and progression of signs and symptoms.
Fundus AF imaging is becoming increasingly important in the examination of many macular diseases, including LMH and MPH. In both, a similar degree of functional loss may occur. In such cases, an increased AF signal at the fovea signifies a loss of foveal tissue that is the macular pigment.
Conflicts of Interest
The author declares that there are no conflicts of interest.
I am greatly indebted to Francois Delori, PhD, for providing the picture of macular pigment distribution in a macaque (Figure 1(b)) and to Roger C. Wagner, PhD, Professor Emeritus of Biological Sciences, University of Delaware, Newark, Delaware, USA, for providing Figure 2(a).
- B. Haouchine, P. Massin, R. Tadayoni, A. Erginay, and A. Gaudric, “Diagnosis of macular pseudoholes and lamellar macular holes by optical coherence tomography,” American Journal of Ophthalmology, vol. 138, no. 5, pp. 732–739, 2004.
- A. J. Witkin, T. H. Ko, J. G. Fujimoto et al., “Redefining lamellar holes and the vitreomacular interface: an ultrahigh-resolution optical coherence tomography study,” Ophthalmology, vol. 113, no. 3, pp. 388–397, 2006.
- J. S. Duker, P. K. Kaiser, S. Binder et al., “The International Vitreomacular Traction Study Group classification of vitreomacular adhesion, traction, and macular hole,” Ophthalmology, vol. 120, no. 12, pp. 2611–2619, 2013.
- A. W. Allen and J. D. M. Gass, “Contraction of a perifoveal epiretinal membrane simulating a macular hole,” American Journal of Ophthalmology, vol. 82, no. 5, pp. 684–691, 1976.
- J. D. M. Gass, “Idiopathic senile macular hole,” Archives of Ophthalmology, vol. 106, no. 5, pp. 629–639, 1988.
- J. D. M. Gass, “Reappraisal of biomicroscopic classification of stages of development of a macular hole,” American Journal of Ophthalmology, vol. 119, no. 6, pp. 752–759, 1995.
- M. R. Hee, C. A. Puliafito, C. Wong et al., “Optical coherence tomography of macular holes,” Ophthalmology, vol. 102, no. 5, pp. 748–756, 1995.
- A. Gaudric, B. Haouchine, P. Massin, M. Paques, P. Blain, and A. Erginay, “Macular hole formation,” Archives of Ophthalmology, vol. 117, no. 6, pp. 744–751, 1999.
- V. Tanner, D. S. Chauhan, T. L. Jackson, and T. H. Williamson, “Optical coherence tomography of the vitreoretinal interface in macular hole formation,” British Journal of Ophthalmology, vol. 85, no. 9, pp. 1092–1097, 2001.
- C. Azzolini, F. Patelli, and R. Brancato, “Correlation between optical coherence tomography data and biomicroscopic interpretation of idiopathic macular hole,” American Journal of Ophthalmology, vol. 132, no. 3, pp. 348–355, 2001.
- B. Haouchine, P. Massin, and A. Gaudric, “Foveal pseudocyst as the first step in macular hole formation,” Ophthalmology, vol. 108, no. 1, pp. 15–22, 2001.
- R. C. Watzke and L. Allen, “Subjective slitbeam sign for macular disease,” American Journal of Ophthalmology, vol. 68, no. 3, pp. 449–453, 1969.
- M. Tsujikawa, M. Ohji, T. Fujikado et al., “Differentiating full thickness macular holes from impending macular holes and macular pseudoholes,” British Journal of Ophthalmology, vol. 81, no. 2, pp. 117–122, 1997.
- F. Bottoni, L. Carmassi, M. Cigada, S. Moschini, and F. Bergamini, “Diagnosis of macular pseudoholes and lamellar macular holes: is optical coherence tomography the “gold standard”?” British Journal of Ophthalmology, vol. 92, no. 5, pp. 635–639, 2008.
- F. Bottoni, A. P. Deiro, A. Giani, C. Orini, M. Cigada, and G. Staurenghi, “The natural history of lamellar macular holes: a spectral domain optical coherence tomography study,” Graefe’s Archive for Clinical and Experimental Ophthalmology, vol. 251, no. 2, pp. 467–475, 2012.
- A. Gaudric, Y. Aloulou, R. Tadayoni, and P. Massin, “Macular pseudoholes with lamellar cleavage of their edge remain pseudoholes,” American Journal of Ophthalmology, vol. 155, no. 4, pp. 733–742, 2013.
- B. Parolini, R. G. Schumann, M. G. Cereda, C. Haritoglou, and G. Pertile, “Lamellar macular hole: a clinicopathologic correlation of surgically excised epiretinal membranes,” Investigative Opthalmology & Visual Science, vol. 52, no. 12, pp. 9074–9083, 2011.
- F. C. Delori, C. K. Dorey, G. Staurenghi et al., “In vivo fluorescence of the ocular fundus exhibits retinal pigment epithelial lipofuscin characteristics,” Investigative Ophthalmology & Visual Science, vol. 36, pp. 718–729, 1995.
- A. von Ruckmann, F. W. Fitzke, and A. C. Bird, “Distribution of fundus autofluorescence with a scanning laser ophthalmoscope,” British Journal of Ophthalmology, vol. 79, no. 5, pp. 407–412, 1995.
- D. M. Snodderly, J. D. Auran, and F. C. Delori, “The macular pigment. II. Spatial distribution in primate retinas,” Investigative Opthalmology & Visual Science, vol. 25, pp. 674–685, 1984.
- S. Syrbe, H. Kuhrt, U. Gärtner et al., “Müller glial cells of the primate foveola: an electron microscopical study,” Experimental Eye Research, vol. 167, pp. 110–117, 2018.
- J. D. M. Gass, “Müller cell cone, an overlooked part of the anatomy of the fovea centralis,” Archives of Ophthalmology, vol. 117, no. 6, pp. 821–823, 1999.
- G. Staurenghi, S. Sadda, U. Chakravarthy, and R. F. Spaide, “Proposed lexicon for anatomic landmarks in normal posterior segment spectral-domain optical coherence tomography,” Ophthalmology, vol. 121, no. 8, pp. 1572–1578, 2014.
- T. Otani, Y. Yamaguchi, and S. Kishi, “Improved visualization of Henle fiber layer by changing the measurement beam angle on optical coherence tomography,” Retina, vol. 31, no. 3, pp. 497–501, 2011.
- B. J. Lujan, A. Roorda, R. W. Knighton, and J. Carroll, “Revealing Henle’s fiber layer using spectral domain optical coherence tomography,” Investigative Opthalmology & Visual Science, vol. 52, no. 3, pp. 1486–1492, 2011.
- A. von Ruckmann, F. W. Fitzke, and Z. J. Gregor, “Fundus autofluorescence in patients with macular holes imaged with a laser scanning ophthalmoscope,” British Journal of Ophthalmology, vol. 82, no. 4, pp. 346–351, 1998.
- A. P. Ciardella, G. C. Lee, K. Langton, J. Sparrow, and S. Chang, “Autofluorescence as a novel approach to diagnosing macular holes,” American Journal of Ophthalmology, vol. 137, no. 5, pp. 956–959, 2004.
- R. dell’Omo, D. Vogt, R. Schumann et al., “The relationship between blue-fundus autofluorescence and optical coherence tomography in eyes with lamellar macular holes,” Investigative Opthalmology & Visual Science, vol. 59, no. 7, pp. 3079–3087, 2018.
- A. Obana, H. Sasano, S. Okazaki, Y. Otsuki, T. Seto, and Y. Gohto, “Evidence of carotenoid in surgically removed lamellar hole-associated epiretinal proliferation,” Investigative Opthalmology & Visual Science, vol. 58, no. 12, pp. 5157–5163, 2017.
Copyright © 2019 Ferdinando Bottoni. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.