Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature

Table 3

The summary of biological corneal decellularization methods discussed in this review.

Biological decellularization methods	Advantages/disadvantages	Significance for future research	References
Enzymes	(i) Often used in decellularization protocols to help remove residual genetic material after cell lysis; (ii) Must be used with another decellularization method; (iii) Can disrupt ECM structure; (iv) Less transparent decellularized corneal tissue	(i) High	[7, 8, 34, 36, 37, 42, 47, 50, 55, 61, 69]
Serum	(i) Efficient decellularization method; (ii) Maintained ultrastructure of decellularized corneal tissue; (iii) Good optical properties after decellularization; (iv) No need for other decellularization methods	(i) High	[61, 70, 71]