Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature

Table 4

The evaluation of efficiency of chemical decellularization methods on corneal tissue and assays used for the evaluation.

Chemical decellularization methods		Decellularization efficiency	Assays used for determination of efficiency of corneal decellularization	References
Surfactants	SDS	+++++	Histological analysis with H&E and DAPI staining	[6, 7, 12, 18, 22, 32, 33, 35, 37, 38, 52]
	SD	++++	Histological analysis with H&E staining	[6, 7],
	Triton X-100	++++/+++++	Histological analysis with H&E and DAPI staining	[2, 35, 37, 39, 41–44]
	CHAPS	+++/++++	Histological analysis with H&E staining, DNA quantification	[6, 35, 35, 45]
Acids		+++	Histological analysis with H&E staining, DNA qualification	[12, 41, 42, 46]
Bases		++	Histological analysis with H&E staining	[41, 42]
EDTA		+++	Histological analysis with H&E and DAPI staining, DNA quantification	[12, 17, 18, 47, 48]

Note. For evaluating efficiency, we used the “+” symbol, where + is the least efficient decellularization method and +++++ is the most efficient decellularization method.