Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature

Table 5

The evaluation of the efficiency of physical decellularization methods on corneal tissue and assays used for the evaluation.

Physical decellularization methods	Efficiency	Assays used for determination of efficiency of corneal decellularization	Sources
Freeze-thaw cycles	+++/++++	Histological analysis with H&E and DAPI staining	[27, 59]
Hypertonic and hypotonic solutions (mostly NaCl)	+++/++++	Histological analysis with H&E and DAPI staining, gel electrophoresis, quantification of DNA	[33, 36, 37, 39, 42–44, 47, 60, 61]
High or ultra-high hydrostatic pressure	++++	Histological analysis with H&E staining, quantification of DNA	[62, 63]
ScCO2	++++	Histological analysis with H & E and DAPI staining, DNA quantificiation	[44, 64–66]

Note. For evaluating efficiency, we used the “+” symbol, where + is the least efficient decellularization method and +++++ is the most efficient decellularization method.