Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature

Table 6

The evaluation of the efficiency of biological decellularization methods on corneal tissue and assays used for the evaluation.

Biological decellularization methods	Efficiency	Assays used for determination of efficiency of corneal decellularization	Sources
Enzymes	++++	Histological analysis with H&E and DAPI staining, gel electrophoresis, quantification of DNA	[7, 36, 37, 42, 47, 55, 61]
Serum	++++/+++++	Histological analysis with H&E and DAPI staining, gel electrophoresis, quantification of DNA	[61, 70, 71]

Note. For evaluating efficiency, we used the “+” symbol, where + is the least efficient decellularization method and +++++ is the most efficient decellularization method.