Restriction Profiling of 23S Microheterogenic Ribosomal Repeats for Detection and Characterizing of E. coli and Their Clonal, Pathogenic, and Phylogroups

Figure 3

(a) Broad range PCR of 23S P2₆₈₂ with genomic DNA isolated from standard strains of E. coli. The upper row comprises Lane 1: K-12 strains; Lane 2: derivatives of K-12 strains; Lane 3: DHI strain; Lane 4: strain C1; Lane 6: Crook’s strain; Lane 7: Walker’s strain; Lane 8: descendent of W; Lane 9: blank. The lower row comprises Lane 1′: B strain; Lane 2′: BL21 strain; Lane 3′: BL21DE3; Lane 4′: BL21DE3Lys5 (B3); Lane 6′: E. coli (ATCC 15223, commensal isolate); Lane 7′: E. coli (ATCC 11775; urine sample); Lane 8′: E. coli (ATCC 25922, clinical). Lanes 5/5′ and Lanes 9/9′ in both rows are 100 bp ladder and blank, respectively. (b) ARDRA of 23S P2₆₈₂ amplicons with Hae III: Lane 1 of both rows comprises 100 bp ladder. The digested products are loaded as per the order given in Figure 2(a).

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