Journal of Parasitology Research

Journal of Parasitology Research / 2015 / Article

Research Article | Open Access

Volume 2015 |Article ID 893752 | 5 pages | https://doi.org/10.1155/2015/893752

Effect of Malathion on Reproductive Parameters of Engorged Female Rhipicephalus (Boophilus) microplus Ticks of Punjab Districts, India

Academic Editor: Bernard Marchand
Received31 Jul 2015
Accepted28 Sep 2015
Published15 Oct 2015

Abstract

The present study was aimed at evaluating effects of malathion on the various reproductive parameters, namely, egg mass weight (EMW), reproductive index (RI), percentage inhibition of oviposition (%IO), and hatchability percentage of eggs of Rhipicephalus (Boophilus) microplus (Canestrini 1887) females from 19 districts of Punjab, India. The effect on various parameters was found to be dose dependent and more discernible upon exposure to higher concentrations. Complete cessation of egg laying was recorded in tick isolates on exposure to 5000 ppm and above. The values of %IO ranged in 4.4–68.6, 25.2–76.2, 35.6–100.0, 45.7–100.0, and 71.4–100.0 in groups treated with 1250, 2500, 5000, 10000, and 20000 ppm of malathion, respectively. A low hatching % was recorded in eggs of all treated female ticks in comparison to control treated with distilled water and complete inhibition of hatching was recorded at 10000 ppm and above. However, the survival of the hatched larvae was not affected and was similar to control group. The results of the current study can be of immense help in formulation and implementation of effective tick control measures.

1. Introduction

One-host cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini 1887), is an economically important ectoparasite of livestock and creates major problem for milk producers in tropical and subtropical countries including India. It causes severe economic losses by blood loss, reduction in weight gain, and direct damage to skin and hides and also by serving as a vector of various economically important infectious diseases [1]. It is the most prevalent tick infesting all age groups of domestic livestock in various agroclimatic zones of Punjab state, India [2, 3]. The global losses due to ticks and tick borne diseases (TTBDs) were estimated to be between US$ 13.9 and 18.7 billion annually [4] while in India the cost of controlling TTBDs has been estimated to be US$ 498.7 million/annum [5].

The control of this parasite is mostly based on the large scale repeated use of chemical acaricides, namely, organophosphates (OP), synthetic pyrethroids (SP), amidines, and macrocyclic lactones (ML) [6]. Pereira et al. [7] reported that to control R. (B.) microplus, it is necessary to consider that only 5% of parasites are located on the host, so the remaining 95% remain in the environment. Accordingly, several studies [810] have emphasized that the successful control of a tick population is related not only to the efficacy of an acaricide but also to the deleterious effects that these active agents cause over tick populations in the field, especially over the reproductive parameters of engorged R. (B.) microplus females.

In India, about 60% of livestock is reared by small and marginal farmers and use of various OP compounds (diazinon and malathion) is very common for the control of livestock and poultry pests [1]. OP compounds are also used against agriculturally important pests and for mass eradication of mosquito larvae in their breeding places [11]. A number of studies have shown development of OP resistance in R. (B.) microplus [12, 13] particularly in Punjab state [14, 15]. However, data on effect of malathion (OP) on the reproductive parameters of ticks indicating its overall tick control efficacy besides causing tick mortality is currently lacking. Based on these observations, the present study aimed to evaluate deleterious effects of malathion on the reproductive parameters of engorged R. (B.) microplus females that had detached from naturally infested cattle.

2. Materials and Methods

2.1. Location, Geography, and Climate of Study Area

Punjab state is located in the northwest region of India which extends from the latitudes 29.30°N to 32.32°N and longitudes 73.55°E to 76.50°E. It covers a geographical area of 50,362 km2 and lies between altitudes 180 and 300 m above sea level. Average rainfall in state is 565.9 mm ranging from 915 mm in north to 102 mm in south with moderately humid climate.

2.2. Collection of Ticks

Fully engorged R. (B.) microplus adult female ticks were collected from the dairy sheds of nineteen districts (Amritsar, Barnala, Bathinda, Faridkot, Fatehgarh Sahib, Ferozepur, Gurdaspur, Hoshiarpur, Jalandhar, Kapurthala, Ludhiana, Mansa, Moga, Muktsar, Pathankot, Patiala, Rupnagar, Sangrur, and SBS Nagar) of Punjab, India. The ticks were collected in separate vials, closed with muslin cloth to allow air and moisture exchange, brought to the Entomology Laboratory, Department of Veterinary Parasitology, College of Veterinary Sciences, GADVASU, Ludhiana, and kept at °C and % relative humidity (RH).

2.3. Acaricide

Technical grade (97.9%) malathion (AccuStandard Inc., USA) was used to prepare the stock solution of 10,000 ppm in methanol. For the bioassay, different concentrations of malathion (1250, 2500, 5000, 10000, and 20000 ppm) were prepared in distilled water from the stock solution and tested against the various field isolates of R. (B.) microplus.

2.4. Adult Immersion Test (AIT)

It was conducted as per the method of Drummond et al. [16]. Briefly, 120 engorged females for each isolate were randomly separated into groups of ten (10). A dose-dependent response study was conducted by immersing adult ticks for two minutes in various concentrations of malathion. Two replicates, with 10 engorged females per replicate, were performed for each concentration. Control ticks were immersed in distilled water. After immersion, the ticks were dried on filter paper with the help of paper towels and placed in sterile Petri dishes for complete drying. Afterwards, the ticks were weighed, were transferred to individual glass tubes covered with muslin cloth, and were kept in incubator maintained at °C and % RH. The adult ticks which survived the exposure of drug laid eggs which were allowed to hatch to larvae under similar conditions of incubation. The ticks which did not oviposit even after 14 days posttreatment were considered dead and the following parameters were compared:(a)Weight of engorged female.(b)Egg mass weight (EMW) laid by the live ticks, recorded at 14 days after treatment.(c)Reproductive index (RI) = egg mass weight/engorged female weight.(d)Percentage inhibition of oviposition (%IO) = [(RI control − RI treated)/RI control × 100].(e)Hatching percentage of eggs.

Dose response data of RI and %IO were analyzed by probit method [17] using GraphPad Prism version 4.0, San Diego, CA, USA. The data were statistically analyzed using a one-way analysis of variance (ANOVA) with group multiple comparisons by Tukey’s test (GraphPad Prism 4).

3. Results and Discussion

The effect of exposure of increasing concentrations of malathion on reproductive parameters of engorged R. (B.) microplus ticks, namely, egg mass weight (EMW), reproductive index (RI), percentage inhibition of oviposition (%IO), and hatching percentage, was studied by AIT. The regression graph of these reproductive parameters of treated ticks was plotted against log values of progressively increasing concentrations of malathion (Table 1). A negative dose-dependent slope was recorded for mean EMW in all tick isolates because with the increasing concentrations of malathion the surviving ticks laid significantly () fewer eggs. Consequently, the mean RI of treated ticks showed a decreasing dose-dependent response and a negative slope was recorded. Results thus indicate that although the increase in concentration of malathion may have not caused one hundred per cent mortality in ticks, the surviving ticks showed a significant decrease () in their efficiency to convert their live weight into egg mass. Also, a dose-dependent significant increase () in the mean %IO of treated ticks along with a positive slope was recorded.


Tick isolatesVariablesSlope ± SE value

AmritsarEMW−27.74 ± 4.020.940.0063
RI−0.24 ± 0.030.930.0074
IO%40.20 ± 6.190.930.0074

BarnalaEMW−27.42 ± 5.380.890.0146
RI−0.17 ± 0.030.890.0153
IO%31.63 ± 6.310.890.0153

BathindaEMW−33.02 ± 7.380.860.0209
RI−0.3375 ± 0.070.860.0222
IO%68.18 ± 15.630.860.0223

FaridkotEMW−23.91 ± 5.320.870.0205
RI−0.24 ± 0.0530.870.0199
IO%40.91 ± 8.990.870.0199

Fatehgarh SahibEMW−39.07 ± 6.100.930.0077
RI−0.37 ± 0.060.920.0088
IO%80.00 ± 13.100.920.0088

FerozepurEMW−15.33 ± 2.530.920.0091
RI−0.16 ± 0.0280.910.0110
IO%34.81 ± 6.150.910.0109

GurdaspurEMW−21.90 ± 10.640.580.1317
RI−0.2336 ± 0.070.780.0453
IO%46.26 ± 13.980.780.0454

HoshiarpurEMW−37.39 ± 6.690.910.0113
RI−0.2951 ± 0.070.840.0272
IO%65.02 ± 14.310.870.0200

JalandharEMW−55.50 ± 7.870.940.0059
RI−0.44 ± 0.060.930.0065
IO%73.37 ± 10.790.930.0065

KapurthalaEMW−22.42 ± 3.900.910.0105
RI−0.23 ± 0.030.920.0091
IO%47.92 ± 7.920.920.0091

LudhianaEMW−14.31 ± 4.880.740.0109
RI−0.12 ± 0.030.810.0368
IO%22.11 ± 6.130.810.0367

MansaEMW−0.03 ± 0.010.790.0417
RI−0.20 ± 0.060.790.0410
IO%0.34 ± 0.100.790.0411

MogaEMW−29.56 ± 2.090.980.0008
RI−0.26 ± 0.010.980.0006
IO%49.11 ± 3.270.980.0006

MuktsarEMW−26.55 ± 4.210.930.0081
RI−0.28 ± 0.050.890.0148
IO%51.42 ± 10.150.890.0148

PathankotEMW−27.03 ± 4.7280.910.0106
RI−0.2924 ± 0.050.910.0114
IO%52.78 ± 9.450.910.0114

PatialaEMW−40.03 ± 11.860.790.0432
RI−0.37 ± 0.110.790.0431
IO%82.31 ± 24.360.790.0431

RupnagarEMW−17.59 ± 2.560.940.0063
RI−0.18 ± 0.020.960.0037
IO%37.63 ± 4.550.960.0037

SangrurEMW−39.36 ± 12.040.780.0468
RI−0.32 ± 0.100.770.0500
IO%0.55 ± 0.170.770.0500

SBS NagarEMW−18.58 ± 4.350.850.0236
RI−0.21 ± 0.030.920.0093
IO%50.28 ± 8.370.920.0093

EMW: egg mass weight; RI: reproductive index; %IO: percentage inhibition of oviposition.

The effect of malathion on the average EMW of treated ticks was recorded to be dose dependent and the decrease was more pronounced at higher concentrations. Complete cessation of egg laying was recorded at 5000 ppm in one isolate, 10000 ppm in four isolates, and 20000 ppm in six isolates, whereas eight isolates laid eggs even upon exposure to the highest concentrations (Table 2). Similarly, details of the effect of malathion on the RI of treated ticks are presented in Table 3. The values of RI ranged from 0.0 to 0.33 in ticks exposed to the recommended concentration of malathion (5000 ppm) used in field conditions. The values of %IO ranged in 4.4–68.6, 25.2–76.2, 35.6–100.0, 45.7–100.0, and 71.4–100.0 in groups treated with 1250, 2500, 5000, 10000, and 20000 ppm of malathion, respectively (Table 4).


IsolateAverage egg mass weight (mg)
Malathion concentration (ppm)Control (DW)
1250250050001000020000

Amritsar29.724.215.00.00.069.9
Barnala55.939.237.322.323.084.2
Bathinda46.831.028.525.00.052.8
Faridkot27.217.63.50.00.051.6
Fatehgarh Sahib41.035.717.50.00.055.2
Ferozepur27.827.317.014.711.049.6
Gurdaspur35.026.026.420.00.050.1
Hoshiarpur35.027.028.516.30.050.1
Jalandhar68.153.222.022.00.077.9
Kapurthala26.814.011.00.00.047.6
Ludhiana20.816.615.515.00.076.9
Mansa65.330.837.318.515.099.6
Moga50.841.233.120.816.565.1
Muktsar45.529.023.516.012.056.5
Pathankot37.421.517.115.00.056.5
Patiala43.334.10.00.00.046.6
Rupnagar29.327.917.013.510.050.4
Sangrur60.930.813.3314.010.072.4
SBS Nagar29.429.014.111.710.042.0


IsolateReproductive index (RI)
Malathion concentration (ppm)Control (DW)
1250250050001000020000

Amritsar0.260.220.140.00.00.61
Barnala0.340.260.260.140.150.53
Bathinda0.470.340.300.270.00.49
Faridkot0.280.190.040.00.00.60
Fatehgarh Sahib0.390.350.170.00.00.47
Ferozepur0.290.290.180.150.120.46
Gurdaspur0.340.230.250.210.00.51
Hoshiarpur0.390.290.330.190.00.51
Jalandhar0.590.420.170.180.00.60
Kapurthala0.280.150.120.00.00.49
Ludhiana0.180.130.120.110.00.56
Mansa0.370.200.260.110.100.60
Moga0.460.400.290.200.150.54
Muktsar0.470.320.180.150.120.56
Pathankot0.410.240.210.170.00.55
Patiala0.410.320.00.00.00.46
Rupnagar0.320.290.190.140.110.49
Sangrur0.530.270.140.150.110.58
SBS Nagar0.350.310.180.130.120.43

Reproductive index (RI) = egg mass weight/engorged female weight.

IsolatePercentage inhibition of oviposition (%IO)
Malathion concentration (ppm)Control (DW)
1250250050001000020000

Amritsar57.264.676.4100.0100.00.0
Barnala36.350.851.173.572.60.0
Bathinda4.431.339.445.7100.00.0
Faridkot54.068.793.8100.0100.00.0
Fatehgarh Sahib16.925.263.9100.0100.00.0
Ferozepur36.637.561.467.474.10.0
Gurdaspur32.953.749.958.8100.00.0
Hoshiarpur22.242.835.682.9100.00.0
Jalandhar8.930.771.169.8100.00.0
Kapurthala42.368.975.0100.0100.00.0
Ludhiana68.676.278.379.9100.00.0
Mansa38.666.856.981.084.00.0
Moga15.426.245.562.371.40.0
Muktsar16.242.767.272.978.50.0
Pathankot26.556.762.968.8100.00.0
Patiala10.930.0100.0100.0100.00.0
Rupnagar35.941.662.570.977.90.0
Sangrur9.153.176.874.981.60.0
SBS Nagar17.927.657.869.672.60.0

%IO = [(RI control − RI treated)/RI control × 100].

The hatching percentage of eggs was determined by visual estimation and a dose-dependent effect was recorded. A low hatching percentage was recorded in eggs laid by all malathion treated female ticks in comparison to control ticks treated with distilled water. Complete inhibition of hatching was recorded in eggs laid by all ticks treated with concentrations of 10000 ppm and above; however, the survival of the hatched larvae was not affected by malathion treatment and was similar to control group.

The absence of studies conducted with the malathion that was used in the present study, regarding its effects on reproductive parameters of fully engorged R. (B.) microplus females, does not allow a comparison with our obtained results. Most trial studies using chemical acaricides are conducted to detect the resistance of R. (B.) microplus to these compounds using in vitro methodologies, such as the Adult Immersion Test (AIT), Larval Packet Test (LPT), or the Larval Immersion Test (LIT), recognized by the Food and Agriculture Organization as a standard for the evaluation of efficacy or resistance [1821].

Further, in the present study, technical grade malathion was selected over commercial formulation for the bioassay because commercial products are prepared with many proprietary ingredients and it is difficult to assess the responses due to active ingredients [22]. For the preparation of stock solution, technical grade malathion was dissolved in 100% methanol and the working concentrations were prepared using water. The use of organic solvents facilitates the adsorption of compound over the surface area of target biological materials and possibly enhances penetration of active ingredients of the acaricide across the exoskeleton [23].

Extensive experience in the field has led to suggestions that the use of strictly managed, uninterrupted, short-interval treatments at recommended concentrations is a reliable means of avoiding or delaying resistance. However, it has also been proposed that intermittent use of high concentration acaricides to kill ticks with resistant alleles may provide a basic means of delaying resistance [24]. Increased concentration has been used successfully in controlling DDT-, OP-, and SP-resistant strains of R. (B.) microplus [25]. This also helped to prolong the life of OP acaricides, but the potential host toxicity and chemical residue problems now need to be reconsidered before an increased concentration could be used for resistance management. However, use of increased concentrations for treatment of animals shed for the elimination of off-the-host stages of the ticks could be beneficial in tick control as they constitute around 95% of total tick population [7]. Several studies [810] have emphasized the successful control of a tick population, due to the deleterious effects that active agents cause over tick populations in the field, especially over the reproductive parameters of engorged R. (B.) microplus females. This would further reduce the number of treatments of the animals and would lead to low residual effects in the milk and meat products thus benefitting the end user health.

Based on this finding, the results that we obtained in this present study regarding the reproductive parameters of fully engorged Rhipicephalus (Boophilus) microplus females might be sufficient to reduce the number of chemical treatments administered to cattle.

Conflict of Interests

The authors declare that there is no conflict of interests.

Acknowledgments

The authors are grateful to Department of Science and Technology, New Delhi, for funding through Women Scientist Scheme (WOS-A) Project no. SR/WOS-A/LS-493/2011 to the senior author. Sincere thanks are also due to the Director of Research, GADVASU, Ludhiana, for providing necessary help.

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