Characterization of Evolutionarily Conserved Trypanosoma cruzi NatC and NatA-N-Terminal Acetyltransferase Complexes

Figure 4

Association of TcNaa30/TcNaa38 and TcNaa10/TcNaa15 with the ribosome. (a) Membrane was incubated with anti-TcNaa30 and anti-TcNaa38. Total cell lysate (T/L), supernatant postultracentrifugation (P/L) and polysomes (Poly) were loaded. As controls, anti-T. cruzi S7 (specific for the ribosome) and anti-T. cruzi cyclophilin A (nonribosomal) were used. Molecular size markers in kDa are indicated on the left. (b) Membrane was incubated with anti-TcNaa10 and TcNaa15. Loading control as mentioned in Figure 4(a).

(a)

(b)