Analysis of the IGS rRNA Region and Applicability for Leishmania (V.) braziliensis Characterization

Table 8

Set of primers specific for Leishmania braziliensis targeting the IGS rRNA region.

Genome location	Primers	Size	% C/G
IGS rRNA	Lb/IGS F 5-CGCACAAAACACACACTGGT-3	20 bases	50%	59.8°C
IGS rRNA	Lb/IGS R 5-TTCATGCTCAGGGACACACTC-3	21 bases	52%	60°C

Legend: IGS—Intergenic Spacer; rRNA—ribosomal RNA; Lb—Leishmania braziliensis; F—forward; R—reverse; % C/G—percentage of cytosine/guanine; —melting temperature.