Electric Cell-Substrate Impedance Sensing of Cellular Effects under Hypoxic Conditions and Carbonic Anhydrase Inhibition

Figure 1

Impedance data, the real part of complex impedance at 10 kHz. (a) Cellular growth. Data are represented as mean ± SD for . (b) Cellular response to normoxia, hypoxia, reoxygenation, and a second stage of hypoxia. Data are represented as mean ± SD for for normoxia, for hypoxia, for hypoxia II, and for reoxygenation. (c) Cellular dynamics under normoxia, normoxia and 100 μM AZA, hypoxia alone, and hypoxia plus AZA. 0.2% DMSO was used as vector control in all experiments. Data are represented as mean ± SD for for normoxia, normoxia and AZA, hypoxia and AZA, and for hypoxia alone. (d) Complementary cell growth assay confirming moderate reduction of cell proliferation under hypoxic conditions (<20%) and AZA inhibition. The results are represented as percentage of normoxia (control), considered 100%. Data are expressed as mean ± SD for .

(a)

(b)

(c)

(d)