Electric Cell-Substrate Impedance Sensing of Cellular Effects under Hypoxic Conditions and Carbonic Anhydrase Inhibition

Figure 5

Extracellular pH changes after 12 hours of cell cultivation in different normoxic and hypoxic conditions. AZA was added just before incubation under normoxia or hypoxia, and the pH of the culture medium was measured from at least four independent experiments. 0.2% DMSO has been used as vector control in all experiments. Data are expressed as mean ± SD for and represent the difference between pH values measured in normoxia, hypoxia, hypoxia with AZA, and normoxia with AZA and the initial pH values.