Table of Contents Author Guidelines Submit a Manuscript
Journal of Spectroscopy
Volume 2016, Article ID 4361051, 13 pages
Research Article

Enamel Based Composite Layers Deposited on Titanium Substrate with Antifungal Activity

1National Institute of Materials Physics, P.O. Box MG 07, 077125 Măgurele, Romania
2Emergency Hospital Floreasca Bucharest, 8 Calea Floresca, 014461 Bucharest, Romania
3Hyperion University of Bucharest, 169 Calea Calarasilor, 030615 Bucharest, Romania
4National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Măgurele, Romania
5Microbiology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, 77206 Bucharest, Romania
6Earth, Environmental and Life Sciences Section, Research Institute of the University of Bucharest, 1–3 Portocalelor Lane, 77206 Bucharest, Romania
7Horiba Jobin Yvon SAS, 16-18 Rue du Canal, 91165 Longjumeau Cedex, France
8Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
9SC METAV Research & Development Bucharest, P.O. Box 22, Bucharest, Romania
10Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Bucharest, Romania
11Faculty of Physics, University of Bucharest, 405 Atomistilor Street, P.O. Box MG 1, 077125 Măgurele, Romania

Received 11 July 2016; Revised 30 September 2016; Accepted 24 October 2016

Academic Editor: Mark Baker

Copyright © 2016 S. L. Iconaru et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The goal of this paper was to investigate the antifungal activity of Enamel layers deposited on titanium substrate (Ti-Enamel) and Enamel layers deposited on titanium substrate previously coated with a vinyl polydimethylsiloxane layer (Ti-PDMS-Enamel). The physicochemical properties were also investigated. The Candida albicans biofilm development on the obtained layers was examined after 24 h, 48 h, and 72 h by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) after ethidium bromide staining. A significant inhibition of the fungal adherence and biofilm development was observed on Ti-Enamel layers. The antifungal results demonstrated that the use of new Ti-Enamel composite layers could represent a promising perspective for the prevention of fungal biofilms associated implant infections.