Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways

Figure 3

Flutamide inhibition of Biz-2 stimulation of PSA mRNA expression. LNCaP cells were induced with Biz-2 in the presence or absence of flutamide, and RNA from induced or uninduced cells was subjected to two-step Taqman real-time RT-PCR. Relative quantitation of PSA mRNA expression was preformed as described in Figure 2. CON: vehicle control; FLU: flutamide; Biz-2: Bizzy nut extract-2; RES: resveratrol; Act.D: actinomycin-D; CHX: cycloheximide. The values are the mean ± SEM of three separate experiments performed in triplicate.