Deoxycholate, an Endogenous Cytotoxin/Genotoxin, Induces the Autophagic Stress-Survival Pathway: Implications for Colon Carcinogenesis
Figure 2
Western blot analysis of LC3-I and LC3-II protein expressions in NCM-460 cells in the presence of protease inhibitors (see Section 2) under
normal conditions, after DOC treatment (a), (c), (d), and under starvation conditions
(e), (g), (h). Bar graphs compare the protein expression of LC3-I and LC3-II over time using computerized densitometric analysis (c), (d), (g), (h). (a), (c), (d) represent LC3-I and LC3-II protein expressions after incubation with 0.4 mM DOC for 30 and
60 minutes compared to control cells that were not incubated with DOC. (e), (g), (h)
represent LC3-I and LC3-II protein expressions at the same time points under
starvation conditions using HBSS. The membranes were stained with Brilliant
Blue G dye to confirm equal protein loading (Figures 2(b), 2(f)). ( indicates
statistically significant (P < .05) differences in mean Relative
Densitometric Units (RDU) between treatment and untreated control groups.)
