Specific Conditions for Resveratrol Neuroprotection against Ethanol-Induced Toxicity

Figure 5

Effect of treatment with 5 μM resveratrol on ethanol-induced nuclear damage in primary cultures of astrocytes. Nuclei were labeled with Hoechst 33342 as described in Section 2. Figure 5 shows representative fluorescent microscope images evidencing the nuclear morphology of astrocytes in control conditions (a), exposed to 5 μM resveratrol for 3 days (b), exposed to 20 mM ethanol for 3 days without (c) or with a recovery period (e), or exposed to 20 mM ethanol for 3 days, with pre- and cotreatment with 5 μM resveratrol (d) and followed by a recovery period (f). In zoom sections of micrographs, arrows point nuclear blebs and nuclear fragmentation. All images are representative fields of at least three independent experiments carried out in duplicate.