Figure 7: NMTMB affected acridine orange (AO) staining of lysosomes of rat hepatocytes over an 8-hour time course. AO was added to hepatocyte media for 10 minutes, removed, and replaced with media containing compound. Treatment with NMTMB proceeded for 30 minutes, 1 h, 2 h, 4 h or 8 h. AO staining was monitored using the Cellomics Arrayscan instrument. The data are expressed as an average of 3 replicates (±SD) from a single cell isolate. A statistically significant difference between control replicates and that for the NMTMB high test concentration is achieved by 30 min ( , -test) where marked cytotoxicity is evident by 4 hr (see Figure 5). Images from the Cellomics at the 30-minute time point are presented in (b).