Table of Contents
Molecular Biology International
Volume 2011, Article ID 876021, 12 pages
http://dx.doi.org/10.4061/2011/876021
Research Article

Antiproliferative, Ultrastructural, and Physiological Effects of Amiodarone on Promastigote and Amastigote Forms of Leishmania amazonensis

1Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas, 373, CCS, Ilha do Fundão, 21941-902 Rio de Janeiro, Brazil
2Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil
3Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
4Instituto Nacional de Metrologia, Normalização e Qualidade Industrial (Inmetro), 20261-232 Rio de Janeiro, Brazil
5Pólo Avançado de Xerém, Universidade Federal do Rio de Janeiro, 25250-470 Rio de Janeiro, Brazil

Received 18 January 2011; Revised 1 March 2011; Accepted 14 March 2011

Academic Editor: Kwang Poo Chang

Copyright © 2011 Sara Teixeira de Macedo-Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Desjeux, “Leishmaniasis: current situation and new perspectives,” Comparative Immunology, Microbiology and Infectious Diseases, vol. 27, no. 5, pp. 305–318, 2004. View at Publisher · View at Google Scholar · View at Scopus
  2. P. D. Marsden and T. C. Jones, “Clinical manifestations, diagnosis, and treatment of leishmaniasis,” in Human Parasitic Diseases, Leishmaniasis, K. P. Chang and R. S. Bray, Eds., vol. 1, pp. 183–198, Elsevier Science, New York, NY, USA, 1985. View at Google Scholar
  3. S. L. Croft, S. Sundar, and A. H. Fairlamb, “Drug resistance in leishmaniasis,” Clinical Microbiology Reviews, vol. 19, no. 1, pp. 111–126, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. S. L. Croft, M. P. Barrett, and J. A. Urbina, “Chemotherapy of trypanosomiases and leishmaniasis,” Trends in Parasitology, vol. 21, no. 11, pp. 508–512, 2005. View at Publisher · View at Google Scholar · View at Scopus
  5. H. Sindermann, S. L. Croft, K. R. Engel et al., “Miltefosine (Impavido): the first oral treatment against leishmaniasis,” Medical Microbiology and Immunology, vol. 193, no. 4, pp. 173–180, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. N. K. Ganguly, “Oral miltefosine may revolutionize treatment of visceral leishmaniasis. The potential impact of miltefosine on visceral leishmaniasis in India,” TDR News, no. 68, p. 2, 2002. View at Google Scholar
  7. J. van Griensven, M. Balasegaram, F. Meheus, J. Alvar, L. Lynen, and M. Boelaert, “Combination therapy for visceral leishmaniasis,” The Lancet Infectious Diseases, vol. 10, no. 3, pp. 184–194, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. G. Benaim, J. M. Sanders, Y. García-Marchan et al., “Amiodarone has intrinsic anti-Trypanosoma cruzi activity and acts synergistically with posaconazole,” Journal of Medicinal Chemistry, vol. 49, no. 3, pp. 892–899, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. X. Serrano-Martín, Y. García-Marchan, A. Fernandez et al., “Amiodarone desestabilizes intracellular Ca2+ homeostasis and biosynthesis of sterols in Leishmania mexicana,” Antimicrobial Agents and Chemotherapy, vol. 53, pp. 1403–1410, 2009. View at Google Scholar
  10. X. Serrano-Martín, G. Payares, M. de Lucca, J. C. Martinez, A. Mendoza-León, and G. Benaim, “Amiodarone and miltefosine act synergistically against Leishmania mexicana and can induce parasitological cure in a murine model of cutaneous leishmaniasis,” Antimicrobial Agents and Chemotherapy, vol. 53, no. 12, pp. 5108–5113, 2009. View at Publisher · View at Google Scholar · View at Scopus
  11. W. E. Courchesne, “Characterization of a novel, broad-based fungicidal activity for the antiarrhythmic drug amiodarone,” Journal of Pharmacology and Experimental Therapeutics, vol. 300, no. 1, pp. 195–199, 2002. View at Publisher · View at Google Scholar · View at Scopus
  12. S. S. Gupta, V.-K. Ton, V. Beaudry, S. Rulli, K. Cunningham, and R. Rao, “Antifungal activity of amiodarone is mediated by disruption of calcium homeostasis,” Journal of Biological Chemistry, vol. 278, no. 31, pp. 28831–28839, 2003. View at Publisher · View at Google Scholar · View at Scopus
  13. A. I. Pozniakovsky, D. A. Knorre, O. V. Markova, A. A. Hyman, V. P. Skulachev, and F. F. Severin, “Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast,” Journal of Cell Biology, vol. 168, no. 2, pp. 257–269, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. N. Dzimiri and A. A. Almotrefi, “Actions of amiodarone on mitochondrial ATPase and lactate dehydrogenase activities in guinea pig heart preparations,” European Journal of Pharmacology, vol. 242, no. 2, pp. 113–118, 1993. View at Google Scholar · View at Scopus
  15. S. M. Ribeiro, A. P. Campello, A. J. Nascimento, and M. L. Kluppel, “Effect of amiodarone (AMD) on the antioxidant enzymes, lipid peroxidation and mitochondrial metabolism,” Cell Biochemistry and Function, vol. 15, no. 3, pp. 145–152, 1997. View at Publisher · View at Google Scholar · View at Scopus
  16. J. W. Card, B. R. Lalonde, E. Rafeiro et al., “Amiodarone-induced disruption of hamster lung and liver mitochondrial function: lack of association with thiobarbituric acid-reactive substance production,” Toxicology Letters, vol. 98, no. 1-2, pp. 41–50, 1998. View at Publisher · View at Google Scholar · View at Scopus
  17. G. Varbiro, A. Toth, A. Tapodi, B. Veres, B. Sumegi, and F. Gallyas, “Concentration dependent mitochondrial effect of amiodarone,” Biochemical Pharmacology, vol. 65, no. 7, pp. 1115–1128, 2003. View at Publisher · View at Google Scholar · View at Scopus
  18. L. G. Warren, “Metabolism of Schizotrypanum cruzi Chagas. I. Effect of culture age and substrate concentration on respiratory rate,” Journal of Parasitology, vol. 46, pp. 529–539, 1960. View at Google Scholar · View at Scopus
  19. N. Sen, B. B. Das, A. Ganguly, T. Mukherjee, S. Bandyopadhyay, and H. K. Majumder, “Camptothecin-induced imbalance in intracellular cation homeostasis regulates programmed cell death in unicellular hemoflagellate Leishmania donovani,” Journal of Biological Chemistry, vol. 279, no. 50, pp. 52366–52375, 2004. View at Publisher · View at Google Scholar · View at Scopus
  20. N. Sen, B. B. Das, A. Ganguly et al., “Camptothecin induced mitochondrial dysfunction leading to programmed cell death in unicellular hemoflagellate Leishmania donovani,” Cell Death and Differentiation, vol. 11, no. 8, pp. 924–936, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. A. Roy, A. Ganguly, S. BoseDasgupta et al., “Mitochondria-dependent reactive oxygen species-mediated programmed cell death induced by 3,3'-diindolylmethane through inhibition of F0F1-ATP synthase in unicellular protozoan parasite Leishmania donovani,” Molecular Pharmacology, vol. 74, no. 5, pp. 1292–1307, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. R. Hofmann, C. Steinwender, J. Kammler, A. Kypta, and F. Leisch, “Effects of a high dose intravenous bolus amiodarone in patients with atrial fibrillation and a rapid ventricular rate,” International Journal of Cardiology, vol. 110, no. 1, pp. 27–32, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. E. E. Golli-Bennour, A. Bouslimi, O. Zouaoui, S. Nouira, A. Achour, and H. Bacha, “Cytotoxicity effects of amiodarone on cultured cells,” Experimental and Toxicologic Pathology. In press.
  24. A. E. Paniz-Mondolfi, A. M. Pérez-Álvarez, O. Reyes-Jaimes et al., “Concurrent Chagas' disease and borderline disseminated cutaneous leishmaniasis: the role of amiodarone as an antitrypanosomatidae drug,” Therapeutics and Clinical Risk Management, vol. 4, no. 3, pp. 659–663, 2008. View at Google Scholar · View at Scopus
  25. A. E. Paniz-Mondolfi, A. M. Pérez-Álvarez, G. Lanza, E. Márquez, and J. L. Concepción, “Amiodarone and itraconazole: a rational therapeutic approach for the treatment of chronic Chagas' disease,” Chemotherapy, vol. 55, no. 4, pp. 228–233, 2009. View at Publisher · View at Google Scholar · View at Scopus
  26. J. C. F. Rodrigues, J. L. Concepcion, C. Rodrigues, A. Caldera, J. A. Urbina, and W. de Souza, “In vitro activities of ER-119884 and E5700, two potent squalene synthase inhibitors, against Leishmania amazonensis: antiproliferative, biochemical, and ultrastructural effects,” Antimicrobial Agents and Chemotherapy, vol. 52, no. 11, pp. 4098–4114, 2008. View at Publisher · View at Google Scholar · View at Scopus
  27. J. C. F. Rodrigues and W. de Souza, “Ultrastructural alterations in organelles of parasitic protozoa induced by different classes of metabolic inhibitos,” Current Pharmaceutical Design, vol. 14, no. 9, pp. 925–938, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. J. C. F. Rodrigues, C. F. Bernardes, G. Visbal, J. A. Urbina, A. E. Vercesi, and W. de Souza, “Sterol methenyl transferase inhibitors alter the ultrastructure and function of the Leishmania amazonensis mitochondrion leading to potent growth inhibition,” Protist, vol. 158, no. 4, pp. 447–456, 2007. View at Publisher · View at Google Scholar · View at Scopus
  29. M. A. Vannier-Santos, J. A. Urbina, A. Martiny, A. Neves, and W. de Souza, “Alterations induced by the antifungal compounds ketoconazole and terbinafine in Leishmania,” Journal of Eukaryotic Microbiology, vol. 42, no. 4, pp. 337–346, 1995. View at Google Scholar · View at Scopus
  30. J. Vivas, J. A. Urbina, and W. de Souza, “Ultrastructural alterations in Trypanosoma (Schizotrypanum) cruzi induced by Δ24(25)-sterol methyltransferase inhibitors and their combi- nations with ketoconazole,” International Journal of Antimicrobial Agents, vol. 7, pp. 235–240, 1996. View at Google Scholar
  31. J. C. F. Rodrigues, M. Attias, C. Rodriguez, J. A. Urbina, and W. de Souza, “Ultrastructural and biochemical alterations induced by 22,26-azasterol, a Δ24(25)-sterol methyltransferase inhibitors, on promastigote and amastigote forms of Leishmania amazonensis,” Antimicrobial Agents and Chemotherapy, vol. 46, no. 2, pp. 487–499, 2002. View at Publisher · View at Google Scholar · View at Scopus
  32. F. Magaraci, C. J. Jimenez, C. Rodrigues et al., “Azasterols as inhibitors of sterol 24-methyltransferase in Leishmania species and Trypanosoma cruzi,” Journal of Medicinal Chemistry, vol. 46, no. 22, pp. 4714–4727, 2003. View at Publisher · View at Google Scholar · View at Scopus
  33. S. O. Lorente, J. C. F. Rodrigues, C. J. Jiménez et al., “Novel azasterols as potential agents for treatment of Leishmaniasis and Trypanosomiasis,” Antimicrobial Agents and Chemotherapy, vol. 48, no. 8, pp. 2937–2950, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. A. C. Granthon, M. V. Braga, J. C. F. Rodrigues et al., “Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors,” Veterinary Parasitology, vol. 146, no. 1-2, pp. 25–34, 2007. View at Publisher · View at Google Scholar · View at Scopus
  35. W. de Souza and J. C. F. Rodrigues, “Sterol biosynthesis pathway as target for anti-trypanosomatid drugs,” Interdisciplinary Perspectives on Infectious Diseases, vol. 2009, Article ID 642502, 19 pages, 2009. View at Publisher · View at Google Scholar
  36. W. E. Courchesne and S. Ozturk, “Amiodarone induces a caffeine-inhibited, MID1-depedent rise in free cytoplasmic calcium in Saccharomyces cerevisiae,” Molecular Microbiology, vol. 47, no. 1, pp. 223–234, 2003. View at Publisher · View at Google Scholar · View at Scopus
  37. E. Krajewska-Kulak and W. Niczyporuk, “Effects of the combination of ketoconazole and calcium channel antagonists against Candida albicans in vitro,” Arzneimittel Forschung, vol. 43, no. 7, pp. 782–783, 1993. View at Google Scholar · View at Scopus
  38. C. O. Rodrigues, R. Catisti, S. A. Uyemura et al., “The sterol composition of Trypanosoma cruzi changes after growth in different culture media and results in different sensitivity to digitonin-permeabilization,” Journal of Eukaryotic Microbiology, vol. 48, no. 5, pp. 588–594, 2001. View at Google Scholar
  39. I. V. Palmié-Peixoto, M. R. Rocha, J. A. Urbina, W. de Souza, M. Einicker-Lamas, and M. C. M. Motta, “Effects of sterol biosynthesis inhibitors on endosymbiont-bearing try panosomatids,” FEMS Microbiology Letters, vol. 255, no. 1, pp. 33–42, 2006. View at Publisher · View at Google Scholar · View at Scopus