Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo
Figure 3
Effect of ubiquinol-10 concentration (a) on CoQ10 levels and (b) the CoQ9/CoQ10 ratio in liver tissues
of mice. Mice were fed with ubiquinol-10 (250 mg/kg/d) or a respective control diet (no ubiquinol-10 supplement) for 7 days
followed by a sublethal intraperitoneal injection with saline (0.9% NaCl, LPS
negative control) for further 4 hours. Thereafter,
liver samples were collected, homogenized, and used for HPLC analysis with
electrochemical detection. CoQ9 was used as an internal standard. Observed CoQ levels were related to the corresponding
protein levels of the respective liver homogenate samples (single data not
shown). All data (one-sided -test) are
means ± SEM of 5 animals in the intervention group (+QH2) and
control group (−QH2), respectively. Protein-related CoQ10 levels and
CoQ9/CoQ10 ratio of ubiquinol-10-supplemented and control animals are depicted in Figures 3(a) and 3(b), respectively.
