In Vitro Inhibition of Neutrophil Elastase Activity by Inhaled Anti-Pseudomonas Antibiotics Used in Cystic Fibrosis Patients

Figure 2

Effect of colistin (a) and (c) and tobramycin (b) and (d) in different concentrations (1, 10, 100, 1000 $\mu$M) on neutrophil elastase activity measurements (neutrophil elastase concentration = 8 $\mu$g/mL) in presence (filled symbols and contineous lines or filled columns) or absence (blank symbols and dashed lines or empty columns) of albumin. In Figures 2(a) and 2(b) change of optical density (OD) over time is given (mean of $n=3$), in Figures 2(c) and 2(d) mean values for slopes of changes in OD over 10 minutes are expressed as means and SEM (bars) and % changes compared to respective control are given of $n=3$. *P <  .05; **P <  .01; ***P <  .001 compared to controls.

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