Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation
Figure 3
MCF-7 cell aggregation is induced by wild-type (WT) CG, but not the enzymatically inactive S195G mutant. (a) MCF-7 cell aggregation assay using CG-overexpressing cell lysates. MCF-7 cells were cultured overnight in medium containing 5% FBS and incubated with lysates serially diluted in serum-free medium containing 1% BSA. After washing, the residual cells were quantified by crystal violet staining. The results are shown as mean SD . When the bars are not shown, they are smaller than the size of the symbols. (b) Immunoreactivities of CG and -actin in the lysates assayed in Figure 3(a). A 10-L aliquot of the whole cell lysate was analyzed by western blotting using anti-CG or -actin antibody. (c) Images of cells that were analyzed in Figure 3(a) at 24 h after incubation with the diluted lysates. Scale bar = 50 m.
