Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation
Figure 5
Binding of CG to the surface of MCF-7 cells is independent of its catalytic site. (a) The S195G CG mutant binds to MCF-7 cells. The cells were incubated with CG-overexpressing RBL-2H3 cell lysate or purified human CG from neutrophils (final concentration, 209 nM) for 90 min on ice. The CG bound to the cell surface was detected by immunostaining with anti-CG antibody in the absence of permeabilization. Scale bar = 10 m. (b) Western blot analysis of CG in the lysates used in Figure 5(a) using anti-CG antibody. A 10-L aliquot of the lysate was loaded in each lane.
