Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid

Figure 4

Effect of CA on the production of NO, TNF-α, and PGE₂, in activated RAW264.7 cells. (a), (b), and (c) Levels of NO, TNF-α, and PGE₂ were determined by the Griess assay, ELISA, and EIA, respectively, from RAW264.7 cell culture supernatants treated with CA (0 to 20 μg/mL) in the presence or absence of peptidoglycan (PGN, 10 μg/mL) (a), pam3CSK (10 μg/mL) (b), or LPS (1 μg/mL) (c), for 6 h (TNF-α) or 24 h (NO and PGE₂). ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared to the control.

(a)

(b)

(c)