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Mediators of Inflammation
Volume 2013, Article ID 216402, 16 pages
Research Article

ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro

1Departamento de Fisiología, Pontificia Universidad Católica de Chile, Alameda 340, 6513677 Santiago, Chile
2Instituto Milenio, Centro Interdisciplinario de Neurociencias de Valparaíso, Pasaje Harrington 287, Playa Ancha, 2360103 Valparaíso, Chile
3Departamento de Fisiología, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Las Condes 12438, 7710162 Santiago, Chile
4Departamento de Neurología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Marcoleta 392, 8330024 Santiago, Chile
5Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA

Received 4 February 2013; Revised 21 March 2013; Accepted 22 March 2013

Academic Editor: Diego Gomez-Nicola

Copyright © 2013 Pablo J. Sáez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Short description for Supplemental Material: Microglia were seeded and after 48 h under control conditions were treated with 1 mM ATP or 1 ng/ml TNF-α, IFN-γ, IL-1β either alone or mixed. Cytokines were added simultaneously and ATP was added 2 h before measurement and is referred as cytokine(s) plus ATP. Treatment with IL-6, IL-1ra, oATP, La3+, Cx43(E2) antibody or 200 µM 10Panx1 were simultaneous to cytokine treatment. We used β-GA for acute GJCs blocking.

  1. Supplementary Materials