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Mediators of Inflammation
Volume 2013, Article ID 245804, 9 pages
Research Article

IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo

1Kennedy Institute of Rheumatology, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7FY, UK
2Leukocyte Biology Section, National Heart and Lung Institute, Sir Alexander Fleming Building, Faculty of Medicine, Imperial College, South Kensington, London SW7 2AZ, UK

Received 31 August 2013; Revised 29 November 2013; Accepted 30 November 2013

Academic Editor: Salahuddin Ahmed

Copyright © 2013 Miriam Weiss et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Pro-inflammatory cytokine expression in M-BMDMs and IRF5 expression in samples used for intracellular IRF5 staining. A. M-CSF differentiated cells were challenged with LPS for the indicated time periods. At each time point RNA (top panel) and supernatants (bottom panel) were collected. Error bars represent the standard error for n=5. These data are the same as in Figure 1A but exclude the GM-CSF data set. B. Irf5 transcript levels were measured by Real-time PCR. Error bars represent the standard deviation of experimental duplicates. Protein levels of IRF5 and β-actin were determined by western blot. RNA and protein samples were from an experiment used to generate data for Figure 2C.

  1. Supplementary Materials