Intricacies for Posttranslational Tumor-Targeted Cytokine Gene Therapy
Figure 5
Not all tumor-targeting peptides can be successfully utilized in this gene delivery method. (a) Multiple tumor-targeting IL12 pDNAs were created by inserting the tumor-targeting peptide-coding sequences into the p40 subunit of the IL12 pDNA as in Figure 1(a). Only the wtIL12, VNTANST-IL12, and CDGRC-IL12 pDNA treatments inhibited primary tumor growth compared to the control pDNA treatments in the 4T1 tumor model. In side-by-side statistical analyses, the inhibition of primary tumor growth with VNTANST-IL12 was significantly inhibited compared to the wtIL12 and CDGRC-IL12 data on day 18 only. (b) Similar results were seen in the development of metastatic tumors in the same mice with only RGD4C not reducing development compared to control. Again, VNTANST-IL12 was significantly different from all groups in side-by-side analyses. Black arrows represent treatments. # represents compared to control. * represents compared to all other groups.
