A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice

Figure 3

ISO at 0.7% preserved lung architecture and normalized lung function in ZY-challenged mice. (a) Lung morphology as assessed by hematoxylin and eosin staining (top, 100x magnification; bottom, 200x magnification). (b) Histologic scoring for the treatment groups in Figure (a). ((c)–(e)) Measurement of markers of lung injury: BALF exudate volume (c), lung wet/dry weight ratio (d), and protein leakage (e). ((f)–(i)) Measurement of markers of lung intactness: PaO₂ (f), PCO₂ (g),HCO₃⁻(h), and pH (i). The animals were treated as shown in Figure 1(a). Data represent means ± SD (𝑛=10mice per group).^∗𝑃<0.05versus sham + vehicle ^#𝑃<0.05versus ZY + vehicle.

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