Long Course Hyperbaric Oxygen Stimulates Neurogenesis and Attenuates Inflammation after Ischemic Stroke
Figure 4
Long course HBO increased neurogenesis. ((a) and (b)) BrdU-NeuN double staining showed that there were significantly more newly forming neurons in the ischemic boundary area (perilesioned cortex (Figure 4(b)) and hippocampus (Figure 4(a))) of HBO3wks rats than HBO2ds rats. (c) Representative images of tissue cytometry using TissueQuest software. BrdU-NeuN positive cells were counted and the signal intensity was quantified. The intensity of co-staining with BrdU and Neu-N was more prominent in HBO3wks group. The difference was significant, as compared with MCAO3wks group and HBO3wks group (). There was still significant difference between HBO2ds group and HBO3wks group (). The difference was even more significant as compared with Sham group and HBO3wks group ().
