Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1, TNF-, IL-6, and HMGB1 Expression
Figure 9
HOCbl modulates eNOS/iNOS, COX-2, HMGB1 gene expression in lung and liver at 24 h subsequent to LPS-induced endotoxaemia. Mice () were treated with HOCbl (0.2 mg/kg i.p low dose regimen (Table 1)) and compared to LPS 0.1 mg/kg (given i.p. at time 0 h). Organs (lung and liver) were harvested at 24 h after LPS and gene expression was quantified in tissue extracts by real-time PCR, using GAPDH and RPL32 as internal standards, and PBS-injected group set as 1. ((a), (b)) eNOS mRNA data; ((c), (d)) iNOS mRNA data; ((e), (f)) COX-2 mRNA data; ((g), (h)) HMGB1 mRNA data. Values are a mean SEM of triplicate observations. versus vehicle (PBS-treated) control; versus LPS group.
