Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin
Figure 3
mRNA expression of NOX1, NOXA1, NOXO1, and p22PHOX in the colon of TNFα-treated mice: effect of apocynin. (a) Mice were treated with TNFα (10 μg · kg−1, ip) in the presence or absence of apocynin (25 mg · kg−1, ip) as described in Figure 1(a) (C in the figure is for control untreated mice). Total RNA from colons was extracted and RT-PCR was performed as described in Section 2 using NOX1, NOXA1, NOXO1, or p22PHOX gene-specific primers. Mice GADPH served as an internal control; (b) densitometric analysis of the ratio of NOX1, NOXA1, NOXO1, or p22PHOX mRNA expression to the total amount of GADPH mRNA. Data are expressed as means ± S.E.M from mice in each group. , , and versus control; , , and versus TNFα.
